| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO24 |
| Uniprot No | O75426 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-580aa |
| 氨基酸序列 | MGEKAVPLLRRRRVKRSCPSCGSELGVEEKRGKGNPISIQLFPPELVEHIISFLPVRDLVALGQTCRYFHEVCDGEGVWRRICRRLSPRLQDQGSGVRPWKRAAILNYTKGLYFQAFGGRRRCLSKSVAPLLAHGYRRFLPTKDHVFILDYVGTLFFLKNALVSTLGQMQWKRACRYVVLCRGAKDFASDPRCDTVYRKYLYVLATREPQEVVGTTSSRACDCVEVYLQSSGQRVFKMTFHHSMTFKQIVLVGQETQRALLLLTEEGKIYSLVVNETQLDQPRSYTVQLALRKVSHYLPHLRVACMTSNQSSTLYVTDQGGVYFEVHTPGVYRDLFGTLQAFDPLDQQMPLALSLPAKILFCALGYNHLGLVDEFGRIFMQGNNRYGQLGTGDKMDRGEPTQVCYLQRPITLWCGLNHSLVLSQSSEFSKELLGCGCGAGGRLPGWPKGSASFVKLQVKVPLCACALCATRECLYILSSHDIEQHAPYRHLPASRVVGTPEPSLGARAPQDPGGMAQACEEYLSQIHSCQTLQDRTEKMKEIVGWMPLMAAQKDFFWEALDMLQRAEGGGGGVGPPAPET |
| 分子量 | 91.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXO24蛋白的3篇假设性参考文献示例(注：因当前可检索数据限制，部分内容可能基于领域知识推测，建议通过学术数据库验证)：
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1. **文献名称**：*Structural and functional characterization of recombinant human FBXO24 protein in the SCF ubiquitin ligase complex*
**作者**：Chen L, et al.
**摘要**：本研究利用大肠杆菌系统表达并纯化了重组人FBXO24蛋白，解析了其与SKP1结合的晶体结构，揭示了其F-box结构域的关键氨基酸对复合体组装的影响，并发现FBXO24通过识别特定底物参与细胞周期调控。
2. **文献名称**：*FBXO24 knockout mice exhibit impaired DNA repair and male infertility*
**作者**：Zhang Y, et al.
**摘要**：通过重组FBXO24蛋白的体外实验，作者发现其与DNA损伤修复蛋白RAD51的相互作用，进一步在基因敲除模型中证实FBXO24缺失导致减数分裂异常，提示其在生殖细胞发育中的关键作用。
3. **文献名称**：*Dysregulation of FBXO24 in glioblastoma promotes tumor invasion via ubiquitination of PTEN*
**作者**：Wang H, et al.
**摘要**：该研究在胶质母细胞瘤中观察到FBXO24的异常高表达，并利用重组蛋白验证了其通过泛素化修饰PTEN蛋白促进肿瘤侵袭的分子机制，为靶向治疗提供了潜在靶点。
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**说明**：以上文献为模拟示例，实际研究可能有限。建议在PubMed、Google Scholar等平台以“FBXO24 recombinant”“FBXO24 function”为关键词检索最新结果。F-box蛋白功能研究通常集中于底物识别及疾病关联，重组蛋白技术常用于互作或结构分析。
The human FBXO24 protein is a member of the F-box protein family, characterized by a conserved F-box domain that enables interaction with SKP1. a core component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. As part of this complex, FBXO24 functions as a substrate-recognition module, targeting specific proteins for ubiquitination and subsequent degradation via the proteasome. While the precise biological roles of FBXO24 remain under investigation, it is implicated in regulating cellular processes such as cell cycle progression, DNA damage response, and stress signaling. Studies suggest its involvement in spermatogenesis, with potential links to male infertility due to its testis-enriched expression. Dysregulation of FBXO24 has also been explored in cancer, where it may act as a tumor suppressor or promoter depending on context, though mechanisms remain unclear. Recombinant human FBXO24 protein, typically produced in bacterial or mammalian expression systems, enables structural and functional studies, including substrate identification, interaction mapping, and enzymatic activity assays. Its availability facilitates research into FBXO24's role in physiological and pathological pathways, potentially aiding therapeutic discoveries for related disorders. Further exploration is required to fully elucidate its regulatory networks and clinical relevance.
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