| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO30 |
| Uniprot No | Q8TB52 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-745aa |
| 氨基酸序列 | MEEELQHSHCVNCVSRRCMTRPEPGISCDLIGCPLVCGAVFHSCKADEHRLLCPFERVPCLNSDFGCPFTMARNKVAEHLEMCPASVVCCTMEWNRWPVSYADRKSYENLSRDVDEVAQLDMALALQDQRMLLESLKVATMMSKATDKVSKPREQISVKSSVPEIPHANGLVSVDEESYGALYQATVETTRSLAAALDILNTATRDIGMLNTSVPNDMDEQQNARESLEDQNLKDQDHLYEEEIGAVGGIDYNDTNQNAQSEQNGSSDLLCDLNTSSYDTSALCNGFPLENICTQVIDQNQNLHGDSKQSNLTNGDCVASSDGTSKPSSSLAVAAQLREIIPSSALPNGTVQHILMPDDEGEGELCWKKVDLGDVKNVDVLSFSHAPSFNFLSNSCWSKPKEDKAVDTSDLEVAEDPMGLQGIDLITAALLFCLGDSPGGRGISDSRMADIYHIDVGTQTFSLPSAILATSTMVGEIASASACDHANPQLSNPSPFQTLGLDLVLECVARYQPKQRSMFTFVCGQLFRRKEFSSHFKNVHGDIHAGLNGWMEQRCPLAYYGCTYSQRRFCPSIQGAKIIHDRHLRSFGVQPCVSTVLVEPARNCVLGLHNDHLSSLPFEVLQHIAGFLDGFSLCQLSCVSKLMRDVCGSLLQSRGMVILQWGKRKYPEGNSSWQIKEKVWRFSTAFCSVNEWKFADILSMADHLKKCSYNVVEKREEAIPLPCMCVTRELTKEGRSLRSVLKPVL |
| 分子量 | 108.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的3篇关于重组人FBXO30蛋白的参考文献示例(实际文献需通过学术数据库核实)：
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1. **文献名称**: *"Expression and Functional Characterization of Recombinant Human FBXO30 in the Ubiquitin-Proteasome System"*
**作者**: Zhang Y. et al. (2020)
**摘要**: 本研究成功在大肠杆菌系统中表达并纯化了重组人FBXO30蛋白，证实其与Skp1蛋白的结合能力，并通过体外泛素化实验证明FBXO30作为SCF复合物底物识别组分，可靶向特定磷酸化修饰的蛋白进行降解。
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2. **文献名称**: *"FBXO30 Mediates Tau Protein Degradation and is Downregulated in Alzheimer's Disease"*
**作者**: Liu X. et al. (2018)
**摘要**: 文章发现重组人FBXO30蛋白通过识别磷酸化修饰的tau蛋白，促进其泛素化依赖的蛋白酶体降解。阿尔茨海默病患者脑组织中FBXO30表达显著降低，提示其可能在神经退行性病变中具有保护作用。
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3. **文献名称**: *"FBXO30 Regulates Cell Cycle Progression by Targeting Cyclin D1 for Ubiquitination"*
**作者**: Wang H. et al. (2021)
**摘要**: 研究利用重组FBXO30蛋白揭示其通过结合并泛素化细胞周期蛋白Cyclin D1.调控G1/S期转换。FBXO30的缺失导致Cyclin D1异常积累，促进癌细胞增殖，表明其作为抑癌因子的潜在机制。
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**备注**：以上文献信息为模拟生成，实际研究请通过PubMed、Web of Science等平台检索最新论文。FBXO30的研究多聚焦于其E3泛素连接酶功能、底物识别机制及在癌症、神经疾病中的病理意义。
Recombinant human FBXO30 protein is a engineered form of the F-box protein 30. a member of the F-box protein family characterized by the presence of a conserved F-box domain. This domain facilitates interactions with SKP1 and CUL1. forming part of the SKP1-CUL1-F-box (SCF) E3 ubiquitin ligase complex, which targets specific substrates for proteasomal degradation. FBXO30 is implicated in regulating cellular processes such as cell cycle progression, apoptosis, and synaptic plasticity. Studies suggest its role in neuronal development and neurodegenerative diseases, with altered expression observed in conditions like Alzheimer's disease and certain cancers.
The recombinant protein is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells), enabling controlled study of its biochemical properties, substrate binding partners, and enzymatic activity. Its purification often involves affinity tags (e.g., His-tag) for isolation and functional validation. Research on recombinant FBXO30 aids in elucidating substrate recognition mechanisms, SCF complex dynamics, and potential therapeutic targeting of ubiquitination pathways. Current investigations focus on identifying physiological substrates and clarifying its dual roles in tumor suppression (via oncoprotein degradation) and neurodegeneration (through abnormal protein aggregation). Its study bridges gaps in understanding post-translational regulation and disease pathogenesis.
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