| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO28 |
| Uniprot No | Q9NVF7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-368aa |
| 氨基酸序列 | MAAAAEERMAEEGGGGQGDGGSSLASGSTQRQPPPPAPQHPQPGSQALPAPALAPDQLPQNNTLVALPIVAIENILSFMSYDEISQLRLVCKRMDLVCQRMLNQGFLKVERYHNLCQKQVKAQLPRRESERRNHSLARHADILAAVETRLSLLNMTFMKYVDSNLCCFIPGKVIDEIYRVLRYVNSTRAPQRAHEVLQELRDISSMAMEYFDEKIVPILKRKLPGSDVSGRLMGSPPVPGPSAALTTMQLFSKQNPSRQEVTKLQQQVKTNGAGVTVLRREISELRTKVQEQQKQLQDQDQKLLEQTQIIGEQNARLAELERKLREVMESAVGNSSGSGQNEESPRKRKKATEAIDSLRKSKRLRNRK |
| 分子量 | 67.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXO28蛋白的3-4条参考文献的示例(**注：以下文献为虚构示例，仅用于格式演示**)：
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1. **文献名称**: *FBXO28 regulates cell cycle progression via targeting cyclin E for ubiquitination*
**作者**: Smith A, et al.
**摘要**: 研究发现重组人FBXO28蛋白作为SCF泛素连接酶复合物的底物识别亚基，通过促进细胞周期蛋白E(cyclin E)的泛素化降解，调控G1/S期转换，其缺失导致细胞周期停滞和基因组不稳定性。
2. **文献名称**: *Structural insights into the interaction of FBXO28 with Skp1 and c-Myc*
**作者**: Zhang L, et al.
**摘要**: 利用重组人FBXO28蛋白进行晶体结构分析，揭示了其F-box结构域与Skp1的结合模式，并发现其C端结构域与致癌转录因子c-Myc相互作用，提示FBXO28可能通过调控c-Myc稳定性参与肿瘤发生。
3. **文献名称**: *FBXO28 overexpression in neuroblastoma promotes tumor growth via NOTCH signaling*
**作者**: Tanaka K, et al.
**摘要**: 研究表明，重组人FBXO28蛋白在神经母细胞瘤中高表达，通过泛素化降解NOTCH通路抑制因子，激活NOTCH信号通路，从而促进肿瘤细胞增殖和侵袭。
4. **文献名称**: *FBXO28 modulates DNA damage response through interaction with ATM kinase*
**作者**: Wang X, et al.
**摘要**: 重组人FBXO28蛋白被证实与ATM激酶相互作用，在DNA损伤修复过程中增强ATM活性，促进下游CHK2磷酸化，调控细胞对辐射损伤的应答能力。
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如需真实文献，建议通过PubMed或Sci-Hub等平台检索关键词“recombinant human FBXO28”、“FBXO28 function”等获取。
**Background of Recombinant Human FBXO28 Protein**
FBXO28 is a member of the F-box protein family, characterized by its conserved F-box domain that facilitates interactions with SKP1. a core component of the SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase complex. This complex targets specific substrates for ubiquitination, marking them for proteasomal degradation. Human FBXO28 contains an N-terminal F-box motif and a C-terminal region with predicted nuclear localization signals, suggesting roles in nuclear processes.
Functionally, FBXO28 has been implicated in regulating cell cycle progression, DNA damage response, and transcriptional control. Studies link it to the stabilization of oncoproteins like MYC, where FBXO28-mediated ubiquitination may fine-tune MYC activity, impacting cell proliferation and tumorigenesis. Dysregulation of FBXO28 has been observed in cancers, highlighting its potential as a therapeutic target.
Recombinant human FBXO28 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, substrate interactions, and enzymatic activity. Purified recombinant FBXO28 enables *in vitro* assays, structural studies, and screening for modulators. Despite progress, its full spectrum of substrates, regulatory mechanisms, and tissue-specific roles remain under exploration, necessitating further research to clarify its physiological and pathological relevance.
This protein is pivotal for dissecting SCF complex dynamics and developing targeted therapies for cancers linked to ubiquitination pathways.
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