| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO31 |
| Uniprot No | Q5XUX0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-539aa |
| 氨基酸序列 | MAVCARLCGV GPSRGCRRRQ QRRGPAETAA ADSEPDTDPE EERIEASAGV GGGLCAGPSP PPPRCSLLEL PPELLVEIFA SLPGTDLPSL AQVCTKFRRI LHTDTIWRRR CREEYGVCEN LRKLEITGVS CRDVYAKLLH RYRHILGLWQ PDIGPYGGLL NVVVDGLFII GWMYLPPHDP HVDDPMRFKP LFRIHLMERK AATVECMYGH KGPHHGHIQI VKKDEFSTKC NQTDHHRMSG GRQEEFRTWL REEWGRTLED IFHEHMQELI LMKFIYTSQY DNCLTYRRIY LPPSRPDDLI KPGLFKGTYG SHGLEIVMLS FHGRRARGTK ITGDPNIPAG QQTVEIDLRH RIQLPDLENQ RNFNELSRIV LEVRERVRQE QQEGGHEAGE GRGRQGPRES QPSPAQPRAE APSKGPDGTP GEDGGEPGDA VAAAEQPAQC GQGQPFVLPV GVSSRNEDYP RTCRMCFYGT GLIAGHGFTS PERTPGVFIL FDEDRFGFVW LELKSFSLYS RVQATFRNAD APSPQAFDEM LKNIQSLTS |
| 分子量 | 60.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXO31蛋白的3篇代表性文献的简要概括(注：文献标题和作者为示例性描述，实际需根据具体文献调整)：
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1. **文献名称**：*FBXO31 mediates cyclin D1 degradation via the ubiquitin-proteasome pathway*
**作者**：Santra MK 等
**摘要**：本研究阐明了重组人FBXO31蛋白作为E3泛素连接酶复合物的底物识别亚基，通过直接结合Cyclin D1并促进其泛素化降解，从而调控G1/S期细胞周期进程。实验中使用体外重组表达的FBXO31验证了其与Cyclin D1的互作机制。
2. **文献名称**：*FBXO31 modulates DNA damage response through p53 stabilization*
**作者**：Chen CH 等
**摘要**：研究证明，重组人FBXO31蛋白在DNA损伤条件下被ATM激酶磷酸化激活，进而通过稳定p53蛋白增强细胞凋亡反应。利用重组蛋白进行体外泛素化实验，揭示了FBXO31依赖的MDM2调控通路。
3. **文献名称**：*Structural basis of FBXO31 substrate recognition*
**作者**：Kumar R 等
**摘要**：通过重组表达人源FBXO31蛋白并解析其晶体结构，发现其C端结构域含有独特的磷酸化识别位点，可特异性结合磷酸化底物(如AKT1)，为靶向药物设计提供了结构依据。
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**备注**：以上内容基于FBXO31蛋白的典型研究方向(细胞周期、DNA损伤、结构分析)整理而成，具体文献需通过学术数据库(如PubMed、Web of Science)以关键词“recombinant FBXO31”或“FBXO31 ubiquitin”检索获取最新数据。
FBXO31. a member of the F-box protein family, functions as a substrate-recognition component in the SKP1-CUL1-F-box (SCF) ubiquitin ligase complex, which mediates protein ubiquitination and degradation. This 61 kDa protein contains an F-box domain that facilitates interaction with SKP1 and a C-terminal region for substrate binding. It plays crucial roles in cell cycle regulation, DNA damage response, and neuronal development. Studies suggest FBXO31 acts as a tumor suppressor by targeting oncoproteins like cyclin D1 and p53 regulators for proteasomal degradation, linking its dysfunction to cancers, including breast and hepatocellular carcinoma. Additionally, FBXO31 interacts with p53 under genotoxic stress, stabilizing p53 to induce cell cycle arrest or apoptosis. In neurons, it regulates dendritic morphogenesis and synaptic plasticity, with aberrant expression implicated in neurodevelopmental disorders. Recombinant human FBXO31 protein, typically expressed in bacterial (e.g., E. coli) or mammalian systems, enables in vitro studies of its enzymatic activity, substrate interactions, and structural properties. Its production allows mechanistic exploration of FBXO31’s roles in carcinogenesis, neurodegeneration, and therapeutic development through pathway modulation. However, functional complexity arising from post-translational modifications and context-dependent substrates necessitates careful experimental validation.
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