| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO36 |
| Uniprot No | Q8NEA4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-188aa |
| 氨基酸序列 | MASWLPETLFETVGQGPPPSKDYYQLLVTRSQVIFRWWKISLRSEYRSTKPGEAKETHEDFLENSHLQGQTALIFGARILDYVINFCKGKFDFLERLSDDLLLTIISYLDLEDIARLCQTSHRFAKLCMSDKLWEQIVQSTCDTITPDVRALAEDTGWRQLFFTNKLQLQRQLRKRKQKYGNLREKQP |
| 分子量 | 48.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人FBXO36蛋白的参考文献概括(部分内容为示例性简化，实际文献可能存在差异)：
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1. **文献名称**：**Structural insights into FBXO36 recruitment for substrate ubiquitination**
**作者**：Li Y, et al.
**摘要**：该研究解析了重组人FBXO36蛋白与SKP1-CUL1复合物的晶体结构，揭示了FBXO36通过F-box结构域与SKP1结合的分子机制，并鉴定了其底物结合区域的氨基酸关键位点，为泛素化底物识别提供了结构基础。
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2. **文献名称**：**FBXO36 regulates cellular protein turnover via Lys48-linked polyubiquitination**
**作者**：Zhang H, et al.
**摘要**：通过重组人FBXO36蛋白表达与体外泛素化实验，研究发现FBXO36能够特异性介导靶蛋白的Lys48位多聚泛素化修饰，促进蛋白酶体降解。功能实验表明，FBXO36缺失导致细胞周期调控蛋白异常累积。
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3. **文献名称**：**Recombinant FBXO36 suppresses neuronal apoptosis in Parkinson’s disease models**
**作者**：Wang X, et al.
**摘要**：本研究利用重组人FBXO36蛋白处理帕金森病细胞模型，发现其通过靶向降解促凋亡蛋白减轻多巴胺能神经元死亡。机制研究表明，FBXO36与α-synuclein存在直接相互作用，可能参与病理蛋白清除。
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4. **文献名称**：**Efficient prokaryotic expression and purification of human FBXO36 for functional screening**
**作者**：Kim S, et al.
**摘要**：文章优化了大肠杆菌中重组人FBXO36蛋白的可溶性表达及纯化方案，验证其体外泛素连接酶活性，并开发基于该蛋白的高通量抑制剂筛选系统，用于靶向药物开发研究。
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**注意**：以上文献为示例性内容，实际文献需通过PubMed/Google Scholar等平台检索确认。如需真实文献，建议以“FBXO36”、“recombinant protein”、“ubiquitination”等关键词查询近年研究。
Recombinant human FBXO36 protein is a ubiquitin ligase component critical in protein degradation regulation. FBXO36 belongs to the F-box protein family, characterized by an F-box domain that binds SKP1 in the SCF (SKP1-Cullin-F-box) E3 ubiquitin ligase complex. This complex targets specific substrates for proteasomal degradation by tagging them with ubiquitin.
Functionally, FBXO36 plays roles in cellular processes such as cell cycle progression, apoptosis, and signal transduction. Emerging studies suggest its involvement in circadian rhythm regulation and neurodegenerative diseases, though its precise substrates and molecular mechanisms remain under investigation. Dysregulation of FBXO36 has been linked to pathological conditions, including cancer and neurological disorders, highlighting its therapeutic potential.
The recombinant form is typically produced in bacterial or mammalian expression systems, enabling biochemical and structural studies. Its applications include in vitro ubiquitination assays, substrate identification, and screening for modulators of ubiquitin ligase activity. Researchers utilize purified FBXO36 to dissect its role in disease pathways and validate interactions with binding partners. Further characterization of FBXO36 could reveal novel drug targets for disorders linked to protein homeostasis imbalances.
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