| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUP133 |
| Uniprot No | Q8WUM0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1156aa |
| 氨基酸序列 | MFPAAPSPRTPGTGSRRGPLAGLGPGSTPRTASRKGLPLGSAVSSPVLFSPVGRRSSLSSRGTPTRMFPHHSITESVNYDVKTFGSSLPVKVMEALTLAEVDDQLTINIDEGGWACLVCKEKLIIWKIALSPITKLSVCKELQLPPSDFHWSADLVALSYSSPSGEAHSTQAVAVMVATREGSIRYWPSLAGEDTYTEAFVDSGGDKTYSFLTAVQGGSFILSSSGSQLIRLIPESSGKIHQHILPQGQGMLSGIGRKVSSLFGILSPSSDLTLSSVLWDRERSSFYSLTSSNISKWELDDSSEKHAYSWDINRALKENITDAIWGSESNYEAIKEGVNIRYLDLKQNCDGLVILAAAWHSADNPCLIYYSLITIEDNGCQMSDAVTVEVTQYNPPFQSEDLILCQLTVPNFSNQTAYLYNESAVYVCSTGTGKFSLPQEKIVFNAQGDSVLGAGACGGVPIIFSRNSGLVSITSRENVSILAEDLEGSLASSVAGPNSESMIFETTTKNETIAQEDKIKLLKAAFLQYCRKDLGHAQMVVDELFSSHSDLDSDSELDRAVTQISVDLMDDYPASDPRWAESVPEEAPGFSNTSLIILHQLEDKMKAHSFLMDFIHQVGLFGRLGSFPVRGTPMATRLLLCEHAEKLSAAIVLKNHHSRLSDLVNTAILIALNKREYEIPSNLTPADVFFREVSQVDTICECLLEHEEQVLRDAPMDSIEWAEVVINVNNILKDMLQAASHYRQNRNSLYRREESLEKEPEYVPWTATSGPGGIRTVIIRQHEIVLKVAYPQADSNLRNIVTEQLVALIDCFLDGYVSQLKSVDKSSNRERYDNLEMEYLQKRSDLLSPLLSLGQYLWAASLAEKYCDFDILVQMCEQTDNQSRLQRYMTQFADQNFSDFLFRWYLEKGKRGKLLSQPISQHGQLANFLQAHEHLSWLHEINSQELEKAHATLLGLANMETRYFAKKKTLLGLSKLAALASDFSEDMLQEKIEEMAEQERFLLHQETLPEQLLAEKQLNLSAMPVLTAPQLIGLYICEENRRANEYDFKKALDLLEYIDEEEDININDLKLEILCKALQRDNWSSSDGKDDPIEVSKDSIFVKILQKLLKDGIQLSEYLPEVKDLLQADQLGSLKSNPYFEFVLKANYEYYVQGQI |
| 预测分子量 | 128 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUP133重组蛋白的3篇代表性文献概览：
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1. **文献名称**：*Structural and Functional Analysis of Nup133 Domains Reveals Modular Assembly of the Nuclear Pore Complex*
**作者**：D'Angelo, M.A.等(2006)
**摘要**：研究解析了NUP133重组蛋白的C端结构域，证明其通过α-螺旋结构参与核孔复合体(NPC)的组装，并调控mRNA出核运输。
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2. **文献名称**：*Mutation in NUP133 Causes Autosomal-Recessive Steroid-Resistant Nephrotic Syndrome*
**作者**：Basel-Vanagaite, L.等(2013)
**摘要**：首次发现NUP133基因突变与常染色体隐性遗传的激素抵抗型肾病综合征相关，重组蛋白功能缺失导致足细胞核孔复合体异常，引发肾小球滤过障碍。
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3. **文献名称**：*Evolutionary Divergence of the Nup155/Nup133 Complex in Trypanosomes*
**作者**：Drinnenberg, I.A.等(2018)
**摘要**：通过重组NUP133与NUP155的互作研究，揭示锥虫中核孔复合体的进化特异性，为开发靶向核运输的寄生虫药物提供依据。
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如需扩展或特定方向文献，可进一步提供关键词或研究背景。
**Background of NUP133 Recombinant Protein**
NUP133 is a critical component of the nuclear pore complex (NPC), a large protein assembly embedded in the nuclear envelope that regulates nucleocytoplasmic transport. As part of the NPC’s structural scaffold, NUP133 belongs to the Nup107-160 subcomplex (Y-complex), which forms the NPC’s outer ring and contributes to its symmetry and stability. This conserved metazoan protein plays a vital role in NPC biogenesis, nuclear envelope organization, and mitotic spindle assembly. Structurally, NUP133 contains a β-propeller domain and an α-solenoid fold, typical of scaffold nucleoporins, enabling interactions with other Y-complex members like NUP107 and NUP160.
Recombinant NUP133 protein is engineered for in vitro studies to dissect its molecular interactions, structural dynamics, and functional contributions to NPC assembly. Due to its role in maintaining nuclear compartmentalization and transport fidelity, dysregulation of NUP133 is linked to diseases, including nephrotic syndrome and certain cancers. Recombinant variants allow researchers to explore pathogenic mutations (e.g., truncations disrupting Y-complex integrity) and their impact on cellular homeostasis.
Additionally, NUP133 recombinant protein aids in structural biology efforts, such as cryo-EM studies, to map NPC architecture. It is also utilized in drug discovery screens targeting nuclear transport pathways and in reconstituting NPC mimics for synthetic biology applications. Studies in model organisms, like zebrafish or mice, rely on recombinant NUP133 to analyze developmental defects caused by NPC dysfunction. Its conserved nature across eukaryotes underscores its fundamental role in cellular biology, making it a pivotal tool for unraveling NPC-related mechanisms in health and disease.
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