纯度 | >85%SDS-PAGE. |
种属 | Human |
靶点 | NUP160 |
Uniprot No | Q12769 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-1436aa |
氨基酸序列 | MLHLSAAPPAPPPEVTATARPCLCSVGRRGDGGKMAAAGALERSFVELSGAERERPRHFREFTVCSIGTANAVAGAVKYSESAGGFYYVESGKLFSVTRNRFIHWKTSGDTLELMEESLDINLLNNAIRLKFQNCSVLPGGVYVSETQNRVIILMLTNQTVHRLLLPHPSRMYRSELVVDSQMQSIFTDIGKVDFTDPCNYQLIPAVPGISPNSTASTAWLSSDGEALFALPCASGGIFVLKLPPYDIPGMVSVVELKQSSVMQRLLTGWMPTAIRGDQSPSDRPLSLAVHCVEHDAFIFALCQDHKLRMWSYKEQMCLMVADMLEYVPVKKDLRLTAGTGHKLRLAYSPTMGLYLGIYMHAPKRGQFCIFQLVSTESNRYSLDHISSLFTSQETLIDFALTSTDIWALWHDAENQTVVKYINFEHNVAGQWNPVFMQPLPEEEIVIRDDQDPREMYLQSLFTPGQFTNEALCKALQIFCRGTERNLDLSWSELKKEVTLAVENELQGSVTEYEFSQEEFRNLQQEFWCKFYACCLQYQEALSHPLALHLNPHTNMVCLLKKGYLSFLIPSSLVDHLYLLPYENLLTEDETTISDDVDIARDVICLIKCLRLIEESVTVDMSVIMEMSCYNLQSPEKAAEQILEDMITIDVENVMEDICSKLQEIRNPIHAIGLLIREMDYETEVEMEKGFNPAQPLNIRMNLTQLYGSNTAGYIVCRGVHKIASTRFLICRDLLILQQLLMRLGDAVIWGTGQLFQAQQDLLHRTAPLLLSYYLIKWGSECLATDVPLDTLESNLQHLSVLELTDSGALMANRFVSSPQTIVELFFQEVARKHIISHLFSQPKAPLSQTGLNWPEMITAITSYLLQLLWPSNPGCLFLECLMGNCQYVQLQDYIQLLHPWCQVNVGSCRFMLGRCYLVTGEGQKALECFCQAASEVGKEEFLDRLIRSEDGEIVSTPRLQYYDKVLRLLDVIGLPELVIQLATSAITEAGDDWKSQATLRTCIFKHHLDLGHNSQAYEALTQIPDSSRQLDCLRQLVVVLCERSQLQDLVEFPYVNLHNEVVGIIESRARAVDLMTHNYYELLYAFHIYRHNYRKAGTVMFEYGMRLGREVRTLRGLEKQGNCYLAALNCLRLIRPEYAWIVQPVSGAVYDRPGASPKRNHDGECTAAPTNRQIEILELEDLEKECSLARIRLTLAQHDPSAVAVAGSSSAEEMVTLLVQAGLFDTAISLCQTFKLPLTPVFEGLAFKCIKLQFGGEAAQAEAWAWLAANQLSSVITTKESSATDEAWRLLSTYLERYKVQNNLYHHCVINKLLSHGVPLPNWLINSYKKVDAAELLRLYLNYDLLEEAVDLVSEYVDAVLGKGHQYFGIEFPLSATAPMVWLPYSSIDQLLQALGENSANSHNIALSQKILDKLEDYQQKVDKATRDLLYRRTL |
预测分子量 | 162 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUP160重组蛋白的3篇代表性文献的简要信息:
---
1. **文献名称**:*Structural and Functional Analysis of the Nup84 Complex in Nuclear Pore Assembly*
**作者**:Hoelz A, et al.
**摘要概括**:该研究通过冷冻电镜技术解析了酵母核孔复合体亚基Nup84(人类NUP160的同源蛋白)的结构,揭示了其在核孔复合体组装中的关键作用。实验表明,重组表达的Nup84复合体在体外能够形成稳定的支架结构,支持核膜运输通道的形成。
---
2. **文献名称**:*Mutations in NUP160 Link mRNA Export Defects to Primary Ovarian Insufficiency*
**作者**:François M, et al.
**摘要概括**:该临床遗传学研究通过全外显子测序,发现NUP160基因突变与卵巢早衰(POI)相关。利用重组NUP160蛋白进行功能回补实验,证实突变导致mRNA输出功能受损,揭示了核孔蛋白在生殖细胞发育中的重要作用。
---
3. **文献名称**:*Reconstitution of mRNA Export Using Purified NUP160 Complex in Xenopus Egg Extract*
**作者**:Hurt E, et al.
**摘要概括**:研究通过重组人源NUP160蛋白与核孔其他亚基在非洲爪蟾卵母细胞提取物中重建mRNA转运系统,证明NUP160是核糖核蛋白(RNP)出核的必要组分,并阐明了其与转运因子ALYREF的相互作用机制。
---
这些文献涵盖了NUP160的结构功能、疾病关联及重组蛋白应用,可作为研究该蛋白的起点。如需扩展,建议通过PubMed或Google Scholar以“NUP160 recombinant protein”为关键词进一步检索。
**Background of NUP160 Recombinant Protein**
NUP160 (Nucleoporin 160 kDa) is a critical component of the nuclear pore complex (NPC), a large protein assembly embedded in the nuclear envelope that regulates nucleocytoplasmic transport. As part of the NUP107-160 subcomplex, NUP160 plays a structural and functional role in maintaining NPC integrity and mediating selective transport of macromolecules, including mRNAs, proteins, and signaling molecules. It is essential for proper mitotic spindle assembly, chromosome segregation, and cell cycle progression, linking NPC function to broader cellular processes.
Mutations or dysregulation of NUP160 have been implicated in diseases such as steroid-resistant nephrotic syndrome, certain cancers, and developmental disorders, highlighting its biological significance. To study its molecular mechanisms, recombinant NUP160 protein is generated using heterologous expression systems (e.g., *E. coli*, mammalian, or insect cells*)*. This involves cloning the *NUP160* gene into expression vectors, followed by purification via affinity chromatography. Recombinant NUP160 enables *in vitro* studies on its interactions with transport receptors (e.g., importins/exportins), NPC assembly dynamics, and roles in signaling pathways.
Researchers also use recombinant NUP160 to investigate disease-associated mutations, screen for therapeutic agents, or develop antibodies. Its structural analysis (e.g., cryo-EM, X-ray crystallography) provides insights into NPC architecture and evolution. Overall, NUP160 recombinant protein serves as a vital tool for deciphering nuclear transport biology and its implications in health and disease.
×