| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CEP110 |
| Uniprot No | O43303 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1012aa |
| 氨基酸序列 | MEEYEKFCEKSLARIQEASLSTESFLPAQSESISLIRFHGVAILSPLLNIEKRKEMQQEKQKALDVEARKQVNRKKALLTRVQEILDNVQVRKAPNASDFDQWEMETVYSNSEVRNLNVPATFPNSFPSHTEHSTAAKLDKIAGILPLDNEDQCKTDGIDLARDSEGFNSPKQCDSSNISHVENEAFPKTSSATPQETLISDGPFSVNEQQDLPLLAEVIPDPYVMSLQNLMKKSKEYIEREQSRRSLRGSINRIVNESHLDKEHDAVEVADCVKEKGQLTGKHCVSVIPDKPSLNKSNVLLQGASTQASSMSMPVLASFSKVDIPIRTGHPTVLESNSDFKVIPTFVTENNVIKSLTGSYAKLPSPEPSMSPKMHRRRSRTSSACHILINNPINACELSPKGKEQAMDLIIQDTDENTNVPEIMPKLPTDLAGVCSSKVYVGKNTSEVKEDVVLGKSNQVCQSSGNHLENKVTHGLVTVEGQLTSDERGAHIMNSTCAAMPKLHEPYASSQCIASPNFGTVSGLKPASMLEKNCSLQTELNKSYDVKNPSPLLMQNQNTRQQMDTPMVSCGNEQFLDNSFEKVKRRLDLDIDGLQKENCPYVITSGITEQERQHLPEKRYPKGSGFVNKNKMLGTSSKESEELLKSKMLAFEEMRKRLEEQHAQQLSLLIAEQEREQERLQKEIEEQEKMLKEKKAMTAEASELDINNAVELEWRKISDSSLLETMLSQADSLHTSNSNSSGFTNSAMQYSFVSANEAPFYLWGSSTSGLTKLSVTRPFGRAKTRWSQVFSLEIQAKFNKITAVAKGFLTRRLMQTDKLKQLRQTVKDTMEFIRSFQSEAPLKRGIVSAQDASLQERVLAQLRAALYGIHDIFFVMDAAERMSILHHDREVRKEKMLRQMDKMKSPRVALSAATQKSLDRKKYMKAAEMGMPNKKFLVKQNPSETRVLQPNQGQNAPVHRLLSRQGTPKTSVKGVVQNRQKPSQSRVPNRVPVSGVYAGKIQRKRPNVATI |
| 预测分子量 | 113.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CEP110重组蛋白的3篇参考文献及其摘要：
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1. **文献名称**：*"CEP110 regulates centrosome maturation and progression through mitosis"*
**作者**：L. Casenghi et al.
**摘要**：该研究通过重组CEP110蛋白的体外功能实验，揭示了CEP110在中心体成熟和有丝分裂进程中的关键作用。实验表明，CEP110通过与Aurora A激酶相互作用调控纺锤体组装，其异常表达会导致染色体分离错误和细胞周期停滞。
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2. **文献名称**：*"Functional characterization of CEP110 overexpression in cancer cells"*
**作者**：M. F. Rousseau & J. W. Kim
**摘要**：文章利用重组CEP110蛋白进行体外肿瘤细胞模型研究，发现CEP110在多种癌症细胞中过表达，并促进细胞异常增殖和迁移。进一步机制研究表明，CEP110通过激活PI3K/AKT信号通路增强肿瘤细胞的侵袭性。
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3. **文献名称**：*"Recombinant CEP110 protein as a tool for studying centrosome amplification"*
**作者**：T. S. Lange & R. Basto
**摘要**：本研究开发了一种高效表达和纯化重组CEP110蛋白的方法，并证明其在体外可诱导中心体过度复制。该蛋白为探索中心体扩增与基因组不稳定性之间的关系提供了重要工具，尤其在癌症发生机制研究中具有应用潜力。
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(注：以上文献信息为示例性内容，实际文献需通过PubMed或学术数据库检索。)
**Background of CEP110 Recombinant Protein**
CEP110 (Centrosomal Protein 110 kDa) is a centrosome-associated protein implicated in regulating centrosome duplication, cell cycle progression, and microtubule organization. The centrosome, a key microtubule-organizing center in animal cells, plays critical roles in cell division, polarity, and intracellular transport. CEP110 localizes to the centrosome and is essential for maintaining its structural integrity and function. It interacts with other centrosomal proteins, such as CDK5RAP2 and pericentrin, to regulate centrosome maturation and spindle formation during mitosis.
CEP110 is encoded by the *CEP110* gene, and its expression is tightly controlled during the cell cycle, peaking at the G1/S transition. Dysregulation of CEP110 has been linked to centrosome amplification, genomic instability, and diseases such as cancer. Overexpression or functional defects in CEP110 may contribute to uncontrolled cell proliferation and tumorigenesis by disrupting normal centrosome duplication cycles.
Recombinant CEP110 protein is engineered using molecular cloning techniques, often expressed in *E. coli* or mammalian systems, and purified for functional studies. It serves as a tool to investigate protein-protein interactions, enzymatic activities, or signaling pathways involving centrosome regulation. Researchers utilize recombinant CEP110 to study its phosphorylation by cell cycle kinases (e.g., CDKs), its role in recruiting other centrosomal components, and its potential as a therapeutic target in cancers with centrosome abnormalities.
Furthermore, studies on CEP110 variants or truncations help elucidate mechanisms underlying developmental disorders and ciliopathies linked to centrosomal dysfunction. By enabling biochemical and structural analyses, recombinant CEP110 advances our understanding of centrosome biology and its implications in disease pathology.
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