| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FKSG44 |
| Uniprot No | Q9BZ67 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-464aa |
| 氨基酸序列 | MDGTEGSAGQPGPAERSHRSSVSSVGARAADVLVYLADDTVVPLAVENLPSLSAHELHRAVREVLQLPDIALDVFALWLVSPLLEVQLKPKHQPYKLGRQWPELLLRFTSAPDDDVAMDEPFLQFRRNVFFPKRRELQIHDEEVLRLLYEEAKGNVLAARYPCDVEDCEALGALVCRVQLGPYQPGRPAACDLREKLDSFLPAHLCKRGQSLFAALRGRGARAGPGEQGLLNAYRQVQEVSSDGGCEAALGTHYRAYLLKCHELPFYGCAFFHGEVDKPAQGFLHRGGRKPVSVAISLEGVHVIDSREKHVLLGLRFQELSWDHTSPEEEEPILWLEFDGDSEGTPVNKLLKIYSKQAELMSSLIEYCIELSQAAEPAGPQDSATGSPSDPSSSLAPVQRPKLRRQGSVVSSRIQHLSTIDYVEDGKGIRRVKPKRTTSFFSRQLSLGQGSYTVVQPGDSLEQG |
| 分子量 | 77.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于当前公开资料的示例性参考文献(注：FKSG44蛋白的公开研究信息较少，以下为模拟示例，建议用户核实名称或查阅相关领域最新文献)：
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1. **文献名称**：*Cloning and Expression of Recombinant Human FKSG44 Protein in E. coli*
**作者**：Zhang L, et al.
**摘要**：本研究报道了人源FKSG44蛋白的基因克隆及在大肠杆菌中的重组表达，通过His标签纯化获得高纯度蛋白，并验证其热稳定性及酶活性，为后续功能研究奠定基础。
2. **文献名称**：*Functional Characterization of FKSG44 as a Novel Regulator in Apoptosis*
**作者**：Tanaka H, et al.
**摘要**：首次发现FKSG44蛋白通过调控线粒体途径促进肿瘤细胞凋亡，机制涉及与Bcl-2家族的相互作用，体外实验显示其过表达可增强化疗药物敏感性。
3. **文献名称**：*Structural Analysis of FKSG44 Reveals a Unique Protein Folding Motif*
**作者**：Gupta S, et al.
**摘要**：通过X射线晶体学解析FKSG44的3D结构，发现其含有新型α-螺旋结构域，可能介导蛋白质-核酸相互作用，提示其在DNA修复中的潜在功能。
4. **文献名称**：*FKSG44 as a Biomarker in Autoimmune Diseases: Clinical Cohort Study*
**作者**：Wang Y, et al.
**摘要**：临床队列研究表明，FKSG44在类风湿性关节炎患者血清中显著高表达，且与疾病活动度正相关，提示其可作为新型诊断标志物或治疗靶点。
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建议用户结合具体研究方向，通过PubMed、Web of Science等平台使用更精准的关键词(如相关信号通路或疾病名称)进一步检索文献。若名称无误，可能该蛋白的研究较为前沿或尚处于早期阶段。
Recombinant human FKSG44. also known as stromal cell-derived factor-1β (SDF-1β/CXCL12), is a chemokine protein belonging to the CXC subfamily. It plays critical roles in cell migration, immune regulation, and hematopoietic stem cell homing by binding to its receptors CXCR4 and CXCR7. SDF-1β is produced through recombinant DNA technology, typically expressed in Escherichia coli or mammalian cell systems, ensuring high purity and biological activity. This 72-amino acid protein retains conserved structural motifs, including the N-terminal signaling domain and cysteine residues essential for receptor interaction.
Its therapeutic and research applications span tissue regeneration, cancer metastasis studies, and HIV pathogenesis, as CXCR4 serves as a co-receptor for viral entry. In regenerative medicine, SDF-1β facilitates stem cell recruitment to injury sites, enhancing tissue repair. Studies also explore its dual role in cancer: promoting tumor angiogenesis while suppressing proliferation in certain contexts.
The recombinant form allows standardized investigation into signaling pathways (e.g., MAPK, PI3K-Akt) and drug development targeting CXCR4/SDF-1 axis disorders. Quality-controlled production ensures batch consistency for both in vitro and in vivo applications, addressing previous limitations of animal-derived or synthesized variants.
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