| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FIP1L1 |
| Uniprot No | Q6UN15 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-594aa |
| 氨基酸序列 | MSAGEVERLV SELSGGTGGD EEEEWLYGGP WDVHVHSDLA KDLDENEVER PEEENASANP PSGIEDETAE NGVPKPKVTE TEDDSDSDSD DDEDDVHVTI GDIKTGAPQY GSYGTAPVNL NIKTGGRVYG TTGTKVKGVD LDAPGSINGV PLLEVDLDSF EDKPWRKPGA DLSDYFNYGF NEDTWKAYCE KQKRIRMGLE VIPVTSTTNK ITAEDCTMEV TPGAEIQDGR FNLFKVQQGR TGNSEKETAL PSTKAEFTSP PSLFKTGLPP SRNSTSSQSQ TSTASRKANS SVGKWQDRYG RAESPDLRRL PGAIDVIGQT ITISRVEGRR RANENSNIQV LSERSATEVD NNFSKPPPFF PPGAPPTHLP PPPFLPPPPT VSTAPPLIPP PGFPPPPGAP PPSLIPTIES GHSSGYDSRS ARAFPYGNVA FPHLPGSAPS WPSLVDTSKQ WDYYARREKD RDRERDRDRE RDRDRDRERE RTRERERERD HSPTPSVFNS DEERYRYREY AERGYERHRA SREKEERHRE RRHREKEETR HKSSRSNSRR RHESEEGDSH RRHKHKKSKR SKEGKEAGSE PAPEQESTEA TPAE |
| 分子量 | 66.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FIP1L1蛋白的3篇假设性参考文献，基于该领域常见研究方向构建。实际文献需通过学术数据库验证：
1. **"Expression and Purification of Recombinant Human FIP1L1 Protein for Structural Studies"**
*作者：Zhang Y, et al.*
摘要：本研究通过大肠杆菌系统成功表达并纯化重组人FIP1L1蛋白，利用圆二色谱和X射线晶体学分析其二级与三级结构，揭示了其N端功能域在RNA结合中的作用。
2. **"Functional Characterization of FIP1L1 in Myeloproliferative Disorders"**
*作者：Cools J, et al.*
摘要：通过重组FIP1L1蛋白与PDGFRA融合基因的功能对比实验，证明FIP1L1的断裂点区域在异常酪氨酸激酶活性激活中的必要性，为靶向治疗提供依据。
3. **"Development of a High-Throughput Assay Using Recombinant FIP1L1-PDGFRA for Drug Screening"**
*作者：Li H, et al.*
摘要：构建重组FIP1L1-PDGFRA融合蛋白，并基于其激酶活性建立药物筛选平台，鉴定出多个小分子抑制剂，潜在用于嗜酸性粒细胞增多综合征的治疗。
**注意**：上述文献为示例性质，实际研究中FIP1L1的研究多聚焦于其与PDGFRA的融合基因(如FIP1L1-PDGFRA在白血病中的作用)。建议通过PubMed或Web of Science以关键词“recombinant FIP1L1”或“FIP1L1-PDGFRA”检索最新文献。
Recombinant human FIP1L1 protein is derived from the FIP1 Like 1 (FIP1L1) gene, located on chromosome 4q12. FIP1L1 encodes a component of the Cleavage and Polyadenylation Specificity Factor (CPSF) complex, which is critical for mRNA 3′-end processing, including polyadenylation—a key step in mRNA maturation and stability. The protein plays a role in regulating gene expression by influencing mRNA export, translation, and degradation.
Interest in FIP1L1 surged after its identification in a chromosomal deletion linked to myeloproliferative disorders. Specifically, the FIP1L1-PDGFRA fusion gene, resulting from a cryptic deletion on 4q12. produces a constitutively active tyrosine kinase implicated in hypereosinophilic syndrome and leukemia. Recombinant FIP1L1 protein enables researchers to study its biochemical properties, interactions (e.g., with PDGFRA or CPSF subunits), and mechanisms underlying pathogenic fusion-driven signaling.
Produced via bacterial or mammalian expression systems, the recombinant protein is utilized in structural studies, in vitro binding assays, and drug discovery efforts targeting FIP1L1-related pathologies. Its availability supports therapeutic research, including inhibitor screening for tyrosine kinase-driven cancers. Understanding FIP1L1’s role in RNA processing and disease mechanisms also contributes to broader insights into post-transcriptional regulation and oncogenesis.
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