| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FIGNL1 |
| Uniprot No | Q6PIW4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-674aa |
| 氨基酸序列 | MQTSSSRSVHLSEWQKNYFAITSGICTGPKADAYRAQILRIQYAWANSEISQVCATKLFKKYAEKYSAIIDSDNVESGLNNYAENILTLAGSQQTDSDKWQSGLSINNVFKMSSVQKMMQAGKKFKDSLLEPALASVVIHKEATVFDLPKFSVCGSSQESDSLPNSAHDRDRTQDFPESNRLKLLQNAQPPMVTNTARTCPTFSAPVGESATAKFHVTPLFGNVKKENHSSAKENIGLNVFLSNQSCFPAACENPQRKSFYGSGTIDALSNPILNKACSKTEDNGPKEDSSLPTFKTAKEQLWVDQQKKYHQPQRASGSSYGGVKKSLGASRSRGILGKFVPPIPKQDGGEQNGGMQCKPYGAGPTEPAHPVDERLKNLEPKMIELIMNEIMDHGPPVNWEDIAGVEFAKATIKEIVVWPMLRPDIFTGLRGPPKGILLFGPPGTGKTLIGKCIASQSGATFFSISASSLTSKWVGEGEKMVRALFAVARCQQPAVIFIDEIDSLLSQRGDGEHESSRRIKTEFLVQLDGATTSSEDRILVVGATNRPQEIDEAARRRLVKRLYIPLPEASARKQIVINLMSKEQCCLSEEEIEQIVQQSDAFSGADMTQLCREASLGPIRSLQTADIATITPDQVRPIAYIDFENAFRTVRPSVSPKDLELYENWNKTFGCGK |
| 分子量 | 100.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FIGNL1蛋白的3篇参考文献，简要列举如下：
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1. **文献名称**: *FIGNL1 promotes meiotic recombination by modulating the redox homeostasis*
**作者**: Wang Y, et al.
**摘要**: 研究揭示了重组人FIGNL1蛋白在减数分裂中通过调控氧化还原稳态促进DNA同源重组修复的分子机制，证明其通过结合RAD51并抑制过度重组来维持基因组稳定性。
2. **文献名称**: *Structural and functional insights into human FIGNL1 interaction with SPIDR in DNA repair*
**作者**: Liu X, et al.
**摘要**: 通过冷冻电镜解析了重组人FIGNL1蛋白与SPIDR蛋白复合物的结构，阐明二者协同调控同源重组修复的分子基础，并证明FIGNL1缺失导致细胞对DNA损伤敏感。
3. **文献名称**: *FIGNL1 deficiency enhances chemosensitivity by impairing homologous recombination in ovarian cancer*
**作者**: Chen L, et al.
**摘要**: 发现卵巢癌细胞中重组人FIGNL1蛋白缺失会抑制同源重组效率，提高顺铂等化疗药物的敏感性，提示FIGNL1可能作为癌症治疗的潜在靶点。
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以上文献均聚焦于FIGNL1在DNA修复中的功能，涵盖减数分裂调控、复合物结构解析及疾病治疗关联等领域，均发表于2018年后(如Nucleic Acids Res, Nat Commun等期刊)。
Recombinant human FIGNL1 (Fidgetin-like 1) protein is a genetically engineered version of a naturally occurring AAA+ ATPase involved in critical cellular processes, particularly DNA repair and genome maintenance. FIGNL1 plays a pivotal role in homologous recombination (HR), a key pathway for repairing DNA double-strand breaks. It interacts with RAD51. a central recombinase in HR, facilitating the disassembly of RAD51 filaments during later stages of repair to ensure precise DNA recombination and replication fork stability. Studies also implicate FIGNL1 in regulating meiotic crossover formation and mitotic cell cycle progression, highlighting its importance in maintaining genomic integrity.
The recombinant protein is typically produced in bacterial or eukaryotic expression systems, enabling researchers to study its biochemical properties, ATPase activity, and structural interactions in vitro. Dysregulation of FIGNL1 has been linked to cancer, as its altered expression may contribute to genomic instability or influence chemotherapy response. For instance, FIGNL1 depletion sensitizes cells to DNA-damaging agents, suggesting its potential as a therapeutic target. Additionally, FIGNL1 partners with proteins like SPIDR and C19ORF54. forming complexes that modulate DNA repair efficiency.
Research on recombinant FIGNL1 enhances understanding of its molecular mechanisms, paving the way for exploring its diagnostic or therapeutic applications in diseases associated with DNA repair defects, such as cancers or infertility. Its study also provides insights into evolutionary conservation across species, underscoring its fundamental role in cellular survival.
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