| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FGR |
| Uniprot No | P09769 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-529aa |
| 氨基酸序列 | MGCVFCKKLEPVATAKEDAGLEGDFRSYGAADHYGPDPTKARPASSFAHIPNYSNFSSQAINPGFLDSGTIRGVSGIGVTLFIALYDYEARTEDDLTFTKGEKFHILNNTEGDWWEARSLSSGKTGCIPSNYVAPVDSIQAEEWYFGKIGRKDAERQLLSPGNPQGAFLIRESETTKGAYSLSIRDWDQTRGDHVKHYKIRKLDMGGYYITTRVQFNSVQELVQHYMEVNDGLCNLLIAPCTIMKPQTLGLAKDAWEISRSSITLERRLGTGCFGDVWLGTWNGSTKVAVKTLKPGTMSPKAFLEEAQVMKLLRHDKLVQLYAVVSEEPIYIVTEFMCHGSLLDFLKNPEGQDLRLPQLVDMAAQVAEGMAYMERMNYIHRDLRAANILVGERLACKIADFGLARLIKDDEYNPCQGSKFPIKWTAPEAALFGRFTIKSDVWSFGILLTELITKGRIPYPGMNKREVLEQVEQGYHMPCPPGCPASLYEAMEQTWRLDPEERPTFEYLQSFLEDYFTSAEPQYQPGDQT |
| 分子量 | 85.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FGR蛋白的3-4篇文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant Human FGR Tyrosine Kinase*
**作者**：Smith, J., et al. (2005)
**摘要**：本研究利用大肠杆菌表达系统成功表达了重组人FGR蛋白，并通过亲和层析纯化获得高纯度产物。功能实验表明，重组FGR具有酪氨酸激酶活性，并能磷酸化下游底物，为后续研究其生物学功能提供了工具。
2. **文献名称**：*Structural Insights into the Kinase Domain of FGR Revealed by X-ray Crystallography*
**作者**：Zhang, L., et al. (2010)
**摘要**：通过X射线晶体学解析了重组人FGR激酶结构域的三维结构，揭示了其活性位点的关键氨基酸残基。研究为设计靶向FGR的小分子抑制剂提供了结构基础，并探讨了其与自身免疫疾病的潜在关联。
3. **文献名称**：*Application of Recombinant FGR in High-Throughput Drug Screening for Leukemia*
**作者**：Johnson, R., et al. (2018)
**摘要**：开发了一种基于重组人FGR蛋白的高通量药物筛选平台，用于鉴定抑制FGR激酶活性的化合物。研究发现多种先导分子可显著抑制白血病细胞的增殖，提示FGR作为治疗靶点的潜力。
4. **文献名称**：*Role of Recombinant FGR in Regulating Macrophage Signaling Pathways*
**作者**：Lee, H., et al. (2020)
**摘要**：通过体外实验证明，重组人FGR蛋白可激活巨噬细胞内的MAPK和NF-κB信号通路，增强促炎细胞因子分泌。该研究支持FGR在炎症反应中的调控作用，为相关疾病机制研究提供了依据。
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**注意**：以上文献信息为虚拟创作，实际研究请查阅PubMed、Web of Science等数据库，或通过“FGR protein recombinant”、“FGR kinase”等关键词检索近年发表的具体论文。
**Background of Recombinant Human FGR Protein**
The FGR protein, encoded by the *FGR* gene (also known as *SRC2*), is a member of the Src family of tyrosine kinases (SFKs). These non-receptor kinases regulate cellular signaling pathways involved in proliferation, differentiation, adhesion, and immune responses. FGR is primarily associated with hematopoietic cells, particularly monocytes, macrophages, and B-cells, where it modulates immune functions and inflammatory processes. Structurally, FGR contains SH2 and SH3 domains critical for protein-protein interactions and a kinase domain responsible for phosphorylating downstream targets.
Aberrant FGR activity is linked to pathological conditions, including leukemia, lymphomas, and chronic inflammatory diseases. Its role in oncogenesis involves dysregulated signaling pathways that promote cell survival, migration, and resistance to apoptosis. In inflammatory contexts, FGR interacts with receptors like FcγR and integrins, influencing phagocytosis and cytokine production.
Recombinant human FGR protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, biologically active forms for research. It serves as a tool to study FGR's molecular mechanisms, screen kinase inhibitors, and explore therapeutic targeting in cancers or immune disorders. Studies also investigate its cross-talk with other SFK members (e.g., SRC, LYN) and its potential as a biomarker for disease progression.
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