| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FGD2 |
| Uniprot No | Q7Z6J4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-655aa |
| 氨基酸序列 | MKGASEEKLASVSNLVTVFENSRTPEAAPRGHRLEDVHHRPECRPPESPGPREKTNVGEAVGSEPRTVSRRYLNSLKNKLSSEAWRKSCQPVTLSGSGTQEPEKKIVQELLETEQAYVARLHLLDQVFFQELLKTARSSKAFPEDVVRVIFSNISSIYQFHSQFFLPELQRRLDDWTANPRIGDVIQKLAPFLKMYSEYVKNFERAAELLATWTDKSPLFQEVLTRIQSSEASGSLTLQHHMLEPVQRIPRYELLLKEYIQKLPAQAPDQADAQKALDMIFSAAQHSNAAITEMERLQDLWEVYQRLGLEDDIVDPSNTLLREGPVLKISFRRNDPMERYLFLFNNMLLYCVPRVIQVGAQFQVRTRIDVAGMKVRELMDAEFPHSFLVSGKQRTLELQARSQEEMISWMQAFQAAIDQIEKRNETFKAAAQGPEGDIQEQELQSEELGLRAPQWVRDKMVTMCMRCQEPFNALTRRRHHCRACGYVVCARCSDYRAELKYDDNRPNRVCLHCYAFLTGNVLPEAKEDKRRGILEKGSSATPDQSLMCSFLQLIGDKWGKSGPRGWCVIPRDDPLVLYVYAAPQDMRAHTSIPLLGYQVTVGPQGDPRVFQLQQSGQLYTFKAETEELKGRWVKAMERAASGWSPSWPNDGDLSD |
| 分子量 | 101.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FGD2蛋白的模拟参考文献示例(均为虚构，仅供格式参考)：
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1. **文献名称**：*Structural and Functional Characterization of Recombinant Human FGD2 Protein*
**作者**：Zhang L, et al.
**摘要**：本研究通过哺乳动物细胞表达系统成功制备了高纯度的重组人FGD2蛋白，并解析了其晶体结构，揭示了其CDC42结合域的关键作用，为探究FGD2在细胞极性调控中的分子机制提供基础。
2. **文献名称**：*FGD2 Modulates Macrophage Immune Responses via CDC42 Signaling*
**作者**：Kim S, Patel R.
**摘要**：文章发现重组FGD2蛋白通过激活CDC42通路调控巨噬细胞炎症因子分泌，提示FGD2在先天免疫及自身免疫性疾病中可能具有潜在治疗价值。
3. **文献名称**：*Expression and Purification of Functional FGD2 in a Bacterial System*
**作者**：Gomez F, et al.
**摘要**：首次报道利用大肠杆菌系统高效表达可溶性重组FGD2蛋白，并通过体外实验验证其鸟嘌呤核苷酸交换因子(GEF)活性，为低成本制备研究级FGD2提供方案。
4. **文献名称**：*FGD2 Deficiency Impairs Osteoclast Differentiation via Rho GTPase Dysregulation*
**作者**：Tanaka Y, et al.
**摘要**：通过重组FGD2蛋白回补实验，证实FGD2缺失导致破骨细胞分化异常，表明其可能通过Rho家族蛋白参与骨代谢疾病的发生。
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注：以上文献为示例性内容，实际研究中请通过PubMed、Web of Science等平台检索真实发表论文。若需具体文献协助，建议提供更详细的研究方向(如FGD2在特定疾病或通路中的作用)。
Facilitator of Glia Maturation-Derived Protein 2 (FGD2) is a member of the FGD family of guanine nucleotide exchange factors (GEFs), which specifically activate the Rho GTPase CDC42. This activation regulates cytoskeletal dynamics, cell polarity, and intracellular signaling pathways. FGD2 is primarily expressed in immune cells, including macrophages and dendritic cells, and plays roles in immune regulation, such as modulating phagocytosis, cytokine production, and cell migration. It also participates in neural development, particularly in myelination processes mediated by Schwann cells. Structurally, FGD2 contains conserved domains critical for its function: a Dbl homology (DH) domain for GEF activity, a pleckstrin homology (PH) domain for membrane targeting, and an FYVE domain for lipid interaction. Dysregulation of FGD2 has been implicated in diseases like cancer, where it may act as either an oncogene or tumor suppressor depending on context. Its dual role highlights tissue-specific signaling complexity. Recombinant human FGD2 protein, produced via expression systems (e.g., E. coli, mammalian cells), enables functional studies to dissect its interactions, downstream pathways, and therapeutic potential. Ongoing research focuses on its application in immunotherapy, neuroregeneration, and targeted cancer therapies.
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