| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FEV |
| Uniprot No | Q99581 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-238aa |
| 氨基酸序列 | MRQSGASQPLLINMYLPDPVGDGLFKDGKNPSWGPLSPAVQKGSGQIQLWQFLLELLADRANAGCIAWEGGHGEFKLTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMSKVHGKRYAYRFDFQGLAQACQPPPAHAHAAAAAAAAAAAAQDGALYKLPAGLAPLPFPGLSKLNLMAASAGVAPAGFSYWPGPGPAATAAAATAALYPSPSLQPPPGPFGAVAAASHLGGHYH |
| 分子量 | 51.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FEV蛋白的参考文献示例(注：部分为虚构条目，供参考格式及内容)：
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1. **标题**: *"Expression and Functional Analysis of Recombinant Human FEV Protein in Neuroendocrine Cells"*
**作者**: Müller A, et al.
**摘要**: 研究通过哺乳动物表达系统成功重组表达了人FEV蛋白，并验证其在神经内分泌细胞中的转录调控功能。实验表明，重组FEV通过结合特定DNA序列抑制促癌基因表达，提示其潜在肿瘤治疗价值。
2. **标题**: *"Purification and Characterization of FEV Protein Produced in E. coli: Implications for Structural Studies"*
**作者**: Kim S, et al.
**摘要**: 利用大肠杆菌系统表达并纯化重组人FEV蛋白，通过质谱和圆二色谱分析确认其正确折叠及稳定性，为后续结构解析和药物靶点开发奠定基础。
3. **标题**: *"FEV as a Novel Regulator of Serotonin Synthesis: Insights from Recombinant Protein-Based Assays"*
**作者**: Patel R, et al.
**摘要**: 研究发现重组FEV蛋白通过调控色氨酸羟化酶(TPH)基因影响血清素合成，揭示FEV在中枢神经系统疾病中的潜在作用机制。
4. **标题**: *"High-Yield Production of Recombinant FEV in HEK293 Cells for Therapeutic Antibody Screening"*
**作者**: Gonzalez L, et al.
**摘要**: 优化HEK293细胞表达体系实现FEV蛋白的高效生产，并利用该重组蛋白筛选出特异性抗体，为靶向FEV的免疫治疗提供工具。
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**说明**：以上文献为示例性质，实际引用时建议通过学术数据库(如PubMed、Web of Science)检索最新且经同行评议的论文。FEV蛋白研究领域相对小众，重点关注其与神经内分泌调控、癌症或单胺代谢相关的文献。
Recombinant human FEV protein is a genetically engineered version of the FEV (Fifth Ewing Variant) protein, a member of the ETS family of transcription factors. The FEV gene, located on human chromosome 2q21. encodes a nuclear protein regulating gene expression by binding to DNA sequences containing ETS motifs. Naturally, FEV plays critical roles in neuroendocrine and immune systems, particularly in serotonin-producing neurons where it governs the expression of tryptophan hydroxylase (TPH), a rate-limiting enzyme in serotonin synthesis. Its dysfunction is linked to mood disorders and neuroendocrine tumors.
Recombinant FEV is produced using expression systems like E. coli or mammalian cells, enabling precise study of its structure-function relationships. The purified protein retains functional domains, including the conserved ETS DNA-binding domain and transcriptional regulatory regions. Applications span basic research (e.g., neurotransmitter regulation, cancer mechanisms) and drug discovery, particularly targeting diseases with disrupted serotonin signaling or ETS-related oncogenesis. Recent studies also explore its role in tumor cell invasion and metastasis, highlighting therapeutic potential. Recombinant FEV tools, including antibodies and assay kits, further support mechanistic investigations in molecular pathology and neuroscience.
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