| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FEZF2 |
| Uniprot No | Q8TBJ5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-459aa |
| 氨基酸序列 | MASSASLETMVPPACPRAGASPATSKTLAFSIERIMAKTSEPRAPFEPRPGALEADGSQGKKLLNLCSPLPCMIPLQPLGYEVPSKTLLSYSELWKSSLRAGGGGGGGGGGGGGGGGAPVCGASGLCKTNCGVCCKAELGLAPSALPAGRVIKPQVINQAVGLTASGSLYYFNYLDSTAYPPSELLSGHLFPSGLLNAQAPAALAAHPKLFLLENAKLAGLAADKFPHPAPYPHKERLPAPLEQVLKENWALTAERGGVKGHSKLPGGSADGKPKNFTCEVCGKVFNAHYNLTRHMPVHTGARPFVCKVCGKGFRQASTLCRHKIIHTQEKPHKCNQCGKAFNRSSTLNTHIRIHAGYKPFVCEFCGKGFHQKGNYKNHKLTHSGEKQYKCTICNKAFHQVYNLTFHMHTHNDKKPFTCATCGKGFCRNFDLKKHVRKLHDSVGPAAPSAKDLTRTVQS |
| 分子量 | 75.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FEZF2蛋白的参考文献示例(注：以下内容为虚构示例，供参考)：
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1. **文献名称**：*FEZF2 modulates neuronal subtype specification through transcriptional regulation*
**作者**：Smith J, et al.
**摘要**：该研究通过体外表达重组人FEZF2蛋白，发现其能特异性激活皮质神经元分化相关基因的启动子。实验表明，FEZF2通过与染色质修饰复合物相互作用，促进神经干细胞向特定神经元亚型的分化，揭示了其在神经发育中的关键调控作用。
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2. **文献名称**：*Crystal structure of the DNA-binding domain of human FEZF2 protein*
**作者**：Chen L, et al.
**摘要**：本研究解析了重组人FEZF2蛋白DNA结合域的晶体结构，揭示了其与靶DNA结合的分子机制。结构分析表明，FEZF2通过保守的锌指基序识别特定序列，为理解其在基因表达调控中的功能提供了结构基础。
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3. **文献名称**：*FEZF2 overexpression drives tumor progression in colorectal cancer*
**作者**：Kim Y, et al.
**摘要**：通过重组FEZF2蛋白的体外功能实验，发现其在结直肠癌细胞中高表达可促进上皮-间质转化(EMT)和转移。机制研究表明，FEZF2通过激活WNT/β-catenin信号通路增强肿瘤细胞的侵袭性，提示其作为潜在治疗靶点。
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4. **文献名称**：*Functional characterization of FEZF2 in human cortical organoid development*
**作者**：Wang H, et al.
**摘要**：利用重组FEZF2蛋白处理人类皮质类器官模型，研究发现FEZF2对脑区特异性神经元模式形成至关重要。敲低FEZF2导致皮质层结构异常，证实其对早期大脑发育的关键调控作用。
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**提示**：以上为示例性内容，实际文献需通过学术数据库(如PubMed、Google Scholar)检索关键词“FEZF2 recombinant protein”或“FEZF2 function”获取。FEZF2的研究多集中于神经发育、肿瘤生物学及转录调控领域。
FeZF2 (FEZ family zinc finger protein 2), also known as ZNF312. is a transcription factor critical for neurodevelopment and cellular differentiation in humans. It belongs to the FEZ protein family characterized by conserved zinc-finger domains that mediate DNA binding and transcriptional regulation. FEZF2 is prominently expressed in the nervous system, where it plays a key role in specifying neuronal subtypes, axon guidance, and cortical layer formation during embryogenesis. Studies highlight its function in regulating genes involved in neural progenitor proliferation, differentiation, and synaptic connectivity.
Beyond neurobiology, FEZF2 has been implicated in cancer progression. Abnormal expression or mutations in FEZF2 are linked to malignancies such as glioblastoma, gastric cancer, and hepatocellular carcinoma, where it may act as an oncogene or tumor suppressor depending on context. Its regulatory effects on cell cycle, apoptosis, and epithelial-mesenchymal transition underscore its multifaceted roles.
Recombinant human FEZF2 protein is engineered for in vitro studies to dissect its molecular mechanisms. Produced via bacterial or mammalian expression systems, it retains DNA-binding activity and enables investigations into its interaction with target promoters, epigenetic modifications, and signaling pathways. This recombinant tool aids in modeling neurodevelopmental disorders, cancer biology, and potential therapeutic targeting. Ongoing research continues to explore FEZF2's clinical relevance in neurodegenerative diseases and oncology.
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