| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FGD3 |
| Uniprot No | Q5JSP0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-725aa |
| 氨基酸序列 | MESGRGSSTPPGPIAALGMPDTGPGSSSLGKLQALPVGPRAHCGDPVSLAAAGDGSPDIGPTGELSGSLKIPNRDSGIDSPSSSVAGENFPCEEGLEAGPSPTVLGAHAEMALDSQVPKVTPQEEADSDVGEEPDSENTPQKADKDAGLAQHSGPQKLLHIAQELLHTEETYVKRLHLLDQVFCTRLTDAGIPPEVIMGIFSNISSIHRFHGQFLLPELKTRITEEWDTNPRLGDILQKLAPFLKMYGEYVKNFDRAVGLVSTWTQRSPLFKDVVHSIQKQEVCGNLTLQHHMLEPVQRVPRYELLLKDYLKRLPQDAPDRKDAERSLELISTAANHSNAAIRKVEKMHKLLEVYEQLGGEEDIVNPANELIKEGQIQKLSAKNGTPQDRHLFLFNSMILYCVPKLRLMGQKFSVREKMDISGLQVQDIVKPNTAHTFIITGRKRSLELQTRTEEEKKEWIQIIQATIEKHKQNSETFKAFGGAFSQDEDPSLSPDMPITSTSPVEPVVTTEGSSGAAGLEPRKLSSKTRRDKEKQSCKSCGETFNSITKRRHHCKLCGAVICGKCSEFKAENSRQSRVCRDCFLTQPVAPESTEKTPTADPQPSLLCGPLRLSESGETWSEVWAAIPMSDPQVLHLQGGSQDGRLPRTIPLPSCKLSVPDPEERLDSGHVWKLQWAKQSWYLSASSAELQQQWLETLSTAAHGDTAQDSPGALQLQVPMGAAAP |
| 分子量 | 86.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人FGD3蛋白的模拟参考文献示例(注：以下内容为生成示例，非真实文献)：
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1. **文献名称**: "Recombinant Human FGD3 Protein Suppresses Tumor Cell Invasion via Regulating Cdc42 Activity"
**作者**: Chen L, et al.
**摘要**: 研究团队成功在大肠杆菌系统中表达重组人FGD3蛋白，并证明其通过激活Cdc42 GTP酶调控肿瘤细胞骨架重塑，抑制乳腺癌细胞侵袭和转移。
2. **文献名称**: "Functional Characterization of FGD3 in Hematopoietic Stem Cell Differentiation"
**作者**: Wang X, et al.
**摘要**: 利用杆状病毒-昆虫细胞系统表达重组FGD3蛋白，发现其通过介导Rho家族信号通路影响造血干细胞的定向分化和白血病细胞凋亡。
3. **文献名称**: "Expression and Purification of Functional FGD3 Protein for Structural Analysis"
**作者**: Müller S, et al.
**摘要**: 优化了哺乳动物细胞表达体系的重组FGD3蛋白纯化方法，结合X射线晶体学解析其结构，揭示了与CDC42结合的特定结构域及其调控机制。
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**说明**：
- FGD3是FGD蛋白家族成员，主要作为鸟苷酸交换因子(GEF)激活CDC42.参与细胞极性、迁移等调控。
- 现有研究多聚焦于其与肿瘤、免疫或发育相关功能，重组蛋白制备是研究其机制的基础步骤。
- 真实文献需通过PubMed、Web of Science等平台以“FGD3 recombinant protein”等关键词检索。
Recombinant human FGD3 (FYVE, RhoGEF, and PH domain-containing protein 3) is a protein derived from the FGD3 gene, which belongs to the FGD family of guanine nucleotide exchange factors (GEFs). FGD3 primarily activates Cdc42. a Rho GTPase involved in cytoskeletal reorganization, cell polarity, and intracellular signaling. Structurally, FGD3 contains multiple functional domains, including a Dbl homology (DH) domain responsible for GEF activity, a pleckstrin homology (PH) domain for membrane targeting, and an FYVE domain for binding phosphatidylinositol lipids. Its expression is associated with tissues such as the brain, liver, and kidneys, as well as immune cells.
Research highlights FGD3's role in regulating cell migration, proliferation, and differentiation, particularly in cancer biology. Studies suggest FGD3 acts as a tumor suppressor, with downregulation observed in breast, gastric, and colorectal cancers. Its loss may promote metastasis by enhancing cytoskeletal dynamics and invasiveness. Recombinant FGD3 protein is typically produced in bacterial or mammalian expression systems, enabling in vitro functional studies, such as analyzing Cdc42 activation pathways or screening for therapeutic agents targeting FGD3-related signaling. Additionally, it supports antibody development for diagnostic or research purposes. Ongoing work aims to clarify its regulatory mechanisms and therapeutic potential in diseases linked to cytoskeletal dysfunction or oncogenesis.
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