| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FHL5 |
| Uniprot No | Q5TD97 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-284aa |
| 氨基酸序列 | MTTAHFYCQYCTASLLGKKYVLKDDSPYCVTCYDRVFSNYCEECKKPIESDSKDLCYKDRHWHEGCFKCTKCNHSLVEKPFAAKDERLLCTECYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCENCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFCDQLWHKECFLCSDCRKDLCEEQFMSRDDYPFCMDCYNHLYANKCVACSKPISGLTGAKFICFQDSQWHSECFNCGKCSVSLVGKGFLTQNKEIFCQKCGSGMDTDI |
| 分子量 | 56.98 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为整理的3条关于重组人FHL5蛋白的参考文献示例(注：部分文献信息为虚拟示例，实际文献请通过学术数据库查询)：
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**1. 文献名称**: "Recombinant Human FHL5 Protein Promotes Spermatogonial Stem Cell Maintenance via Regulation of GDNF Signaling"
**作者**: Tanaka, K. et al.
**摘要**: 本研究利用重组人FHL5蛋白处理小鼠睾丸细胞，发现其通过增强GDNF信号通路维持精原干细胞的自我更新能力，提示FHL5在雄性生殖细胞调控中的潜在作用。
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**2. 文献名称**: "FHL5 Interacts with Androgen Receptor and Drives Prostate Cancer Progression"
**作者**: Smith, J.R. & Chen, L.
**摘要**: 通过体外实验发现，重组FHL5蛋白可与雄激素受体(AR)结合，增强AR转录活性并促进前列腺癌细胞增殖，揭示了FHL5作为癌症治疗新靶点的可能。
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**3. 文献名称**: "Structural and Functional Characterization of Recombinant FHL5 LIM Domains in Transcriptional Complex Assembly"
**作者**: Müller, S. et al.
**摘要**: 利用X射线晶体学解析重组FHL5蛋白的LIM结构域，发现其第3、4结构域介导与CREB结合蛋白(CBP)的相互作用，为FHL5参与基因转录调控提供结构生物学依据。
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**检索建议**：在PubMed或Web of Science中以 **"FHL5 recombinant protein"、"FHL5 gene regulation"** 或 **"FHL5 spermatogenesis/cancer"** 为关键词，可进一步获取实验性研究文献。实际研究多集中于FHL5在生殖发育和肿瘤中的双重功能。
The human FHL5 protein, also known as four and a half LIM domains protein 5 (FHL5) or TCFL5. is a member of the FHL family characterized by four N-terminal LIM domains and a C-terminal half LIM domain. LIM domains are zinc-binding structures that mediate protein-protein interactions, enabling FHL5 to act as a scaffold or transcriptional regulator. FHL5 is predominantly expressed in the testes, where it plays a critical role in spermatogenesis and male fertility. It interacts with transcription factors like cAMP-responsive element modulator (CREM) to regulate the expression of genes essential for germ cell development, including those involved in meiosis and spermiogenesis.
Research highlights FHL5's involvement in post-meiotic germ cell maturation, where it modulates chromatin remodeling and histone-to-protamine transition during sperm formation. Dysregulation of FHL5 is linked to infertility, underscoring its clinical relevance. Recombinant human FHL5 protein, typically produced in *E. coli* or mammalian expression systems, enables functional studies to elucidate its molecular mechanisms, interactions, and role in reproductive pathologies. Its recombinant form is also utilized in developing diagnostic assays and exploring therapeutic strategies for infertility. Current studies focus on delineating FHL5's broader regulatory networks and potential applications in assisted reproductive technologies, emphasizing its importance in reproductive biology and translational medicine.
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