| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FIGNL2 |
| Uniprot No | A6NMB9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-653aa |
| 氨基酸序列 | MHWTPEHAQPLNQWPEQHLDVSSTTPSPAHKLELPPGGRQRCHYAWAHDDISALTASNLLKRYAEKYSGVLDSPYERPALGGYSDASFLNGAKGDPEPWPGPEPPYPLASLHEGLPGTKSGGGGGSGALGGSPVLAGNLPEPLYAGNACGGPSAAPEYAAGYGGGYLAPGYCAQTGAALPPPPPAALLQPPPPPGYGPSAPLYNYPAGGYAAQPGYGALPPPPGPPPAPYLTPGLPAPTPLPAPAPPTAYGFPTAAPGAESGLSLKRKAADEGPEGRYRKYAYEPAKAPVADGASYPAADNGECRGNGFRAKPPGAAEEASGKYGGGVPLKVLGSPVYGPQLEPFEKFPERAPAPRGGFAVPSGETPKGVDPGALELVTSKMVDCGPPVQWADVAGQGALKAALEEELVWPLLRPPAYPGSLRPPRTVLLFGPRGAGKALLGRCLATQLGATLLRLRGATLAAPGAAEGARLLQAAFAAARCRPPSVLLISELEALLPARDDGAAAGGALQVPLLACLDGGCGAGADGVLVVGTTSRPAALDEATRRRFSLRFYVALPDSPARGQILQRALAQQGCALSERELAALVQGTQGFSGGELGQLCQQAAAGAGLPGLQRPLSYKDLEAALAKVGPRASAKELDSFVEWDKMYGSGH |
| 分子量 | 98.23 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FIGNL2蛋白的3条示例参考文献(注：部分信息为示例性虚构，建议通过学术数据库核实实际文献)：
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1. **文献名称**：**"FIGNL2 interacts with RAD51 to orchestrate homologous recombination and suppress genome instability"**
**作者**：Li, Y., et al.
**摘要**：研究表明，重组人FIGNL2蛋白通过其AAA+ ATP酶活性与RAD51直接结合，调控同源重组(HR)修复过程中的RAD51纤丝动态。敲低FIGNL2导致DNA损伤修复缺陷，增加染色体异常和细胞凋亡，提示其在维持基因组稳定性中的关键作用。
2. **文献名称**：**"Recombinant human FIGNL2 expression and biochemical characterization of its ATP-dependent DNA binding activity"**
**作者**：Sakamoto, T., et al.
**摘要**：该研究成功在大肠杆菌系统中表达并纯化重组人FIGNL2蛋白，发现其具有ATP依赖性微管结合能力。体外实验进一步证明，FIGNL2通过水解ATP调控微管动力学，可能影响细胞分裂过程中的纺锤体组装。
3. **文献名称**：**"CRISPR screens identify FIGNL2 as a guardian of meiotic recombination fidelity"**
**作者**：Chen, X., et al.
**摘要**：通过全基因组CRISPR筛选，发现FIGNL2缺失导致减数分裂过程中同源重组修复异常，出现未修复的DNA双链断裂(DSB)。研究揭示FIGNL2与DMC1协同作用，确保减数分裂交叉形成的精确性。
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**注**：以上内容仅为示例，真实文献需通过PubMed、Web of Science等平台检索确认。推荐关键词：**"FIGNL2 recombination"**、**"FIGNL2 RAD51"**、**"FIGNL2 DNA repair"**。
Fidgetin-like protein 2 (FIGNL2) is an evolutionarily conserved AAA+ ATPase involved in genome maintenance and DNA repair. As a member of the fidgetin family, it shares structural homology with the canonical fidgetin protein but exhibits distinct functional roles. FIGNL2 is primarily associated with homologous recombination (HR) repair, a critical pathway for resolving DNA double-strand breaks (DSBs). Studies suggest that FIGNL2 interacts with key HR mediators, such as RAD51 and BRCA2. facilitating the dynamic assembly and disassembly of RAD51 nucleoprotein filaments during strand exchange. This activity ensures precise repair fidelity and prevents genomic instability.
In meiosis, FIGNL2 participates in crossover formation and chromosome segregation, underscoring its importance in gametogenesis. Dysregulation of FIGNL2 has been linked to replication stress sensitivity, premature aging, and tumorigenesis, highlighting its dual role in cellular homeostasis and disease. Recombinant human FIGNL2 (rhFIGNL2) is produced via heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Its purified form enables biochemical analyses, including ATPase activity assays and in vitro DNA-protein interaction studies.
Current research focuses on elucidating FIGNL2's structural motifs, such as its conserved AAA+ domain, and exploring its therapeutic potential in cancer treatment, particularly in HR-deficient cancers. Understanding FIGNL2's molecular mechanisms may advance strategies to enhance DNA repair precision or sensitize cancer cells to genotoxic therapies.
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