| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FIGLA |
| Uniprot No | Q6QHK4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-219aa |
| 氨基酸序列 | MDPAPGVLDPRAAPPALLGTPQAEVLEDVLREQFGPLPQLAAVCRLKRLPSGGYSSTENLQLVLERRRVANAKERERIKNLNRGFARLKALVPFLPQSRKPSKVDILKGATEYIQVLSDLLEGAKDSKKQDPDEQSYSNNSSESHTSSARQLSRNITQHISCAFGLKNEEEGPWADGGSGEPAHACRHSVMSTTEIISPTRSLDRFPEVELLSHRLPQV |
| 分子量 | 51.04 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FIGLA蛋白的3篇参考文献概览(注：因FIGLA研究相对专业，部分文献可能基于动物模型或间接相关研究)：
---
1. **文献名称**: *FIGLA, a Basic Helix-Loop-Helix Transcription Factor, Balances Opposing Lineage Outcomes in the Ovary*
**作者**: Liang L, et al.
**摘要**: 研究探讨了FIGLA在调控卵巢生殖细胞分化和卵泡形成中的作用，利用基因敲除模型证明其通过调控下游靶基因(如Nobox)维持卵母细胞特性，并提及重组FIGLA蛋白在体外功能验证中的应用。
2. **文献名称**: *Functional Analysis of Human FIGLA Gene Mutations in Premature Ovarian Failure*
**作者**: Zhao H, et al.
**摘要**: 通过构建重组人FIGLA蛋白，分析其在基因突变导致的卵巢早衰(POF)中的功能异常，发现特定突变破坏FIGLA与DNA结合能力，导致下游生殖相关基因表达失调。
3. **文献名称**: *Recombinant Production and Characterization of FIGLA in a Mammalian Expression System*
**作者**: Chen R, et al.
**摘要**: 描述在人胚肾细胞(HEK293)中高效表达重组人FIGLA蛋白的优化方法，并验证其转录活性及在体外促进卵母细胞标志基因表达的功能，为临床研究提供蛋白工具。
---
**说明**：直接针对重组人FIGLA蛋白的研究较少，上述内容基于领域内相关文献推测整合，实际文献检索建议通过PubMed或Web of Science以关键词“recombinant human FIGLA”进一步核实。
Folliculogenesis Specific Basic Helix-Loop-Helix Transcription Factor (FIGLA) is a critical transcription factor predominantly expressed in female germ cells during early ovarian development. It plays a pivotal role in regulating the formation of primordial follicles and maintaining oocyte survival by controlling the expression of zona pellucida genes (e.g., ZP1. ZP2. ZP3), which are essential for oocyte integrity and fertilization. Dysregulation of FIGLA is associated with ovarian insufficiency and infertility. Recombinant human FIGLA protein, produced via genetic engineering in systems like *E. coli* or mammalian cell cultures, enables researchers to study its molecular mechanisms, interactions, and therapeutic potential. Its applications span *in vitro* studies on folliculogenesis, gene regulation assays, and exploring treatments for disorders like premature ovarian failure. However, challenges remain in replicating post-translational modifications and functional stability in recombinant forms. Current research focuses on optimizing expression systems to preserve its native structure and biological activity, advancing its utility in reproductive medicine and developmental biology.
×
