| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ00060 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-78aa |
| 氨基酸序列 | MANTEPTEGQRTDEEEPAAEETQEIIYAQLNHQALSQTGFPPASQCPHYLSEDPSIYITVHQAQAEARAAPSLWHKGH |
| 分子量 | 35.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是一些关于重组人FLJ00060蛋白的参考文献示例(注：文献为虚构示例，实际需根据真实数据库检索补充)：
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1. **文献名称**: "Recombinant Human FLJ00060 Protein Enhances Wnt/β-Catenin Signaling in Stem Cells"
**作者**: Zhang Y, et al.
**摘要**: 研究报道了重组人FLJ00060蛋白通过激活Wnt/β-catenin信号通路促进胚胎干细胞的自我更新能力，并解析了其与LRP6受体的相互作用机制。
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2. **文献名称**: "Expression and Purification of FLJ00060 in HEK293 Cells: Implications for Cancer Therapy"
**作者**: Thompson R, et al.
**摘要**: 本文优化了FLJ00060在HEK293系统中的重组表达与纯化工艺，发现该蛋白可抑制结直肠癌细胞增殖，提示其潜在的肿瘤治疗应用价值。
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3. **文献名称**: "Structural Characterization of FLJ00060 and Its Role in MAPK Pathway Regulation"
**作者**: Suzuki M, et al.
**摘要**: 通过X射线晶体学解析了FLJ00060的三维结构，揭示了其通过结合MAPK激酶调控细胞应激反应的分子机制。
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4. **文献名称**: "FLJ00060 Knockout Mice Exhibit Impaired Angiogenesis and Cardiac Development"
**作者**: Chen L, et al.
**摘要**: 利用基因敲除模型发现，FLJ00060缺失导致小鼠胚胎期血管生成障碍和心脏发育异常，证实其在血管内皮细胞迁移中的关键作用。
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如需获取真实文献，建议通过PubMed或Google Scholar检索“FLJ00060”或“C14orf159”(可能为该基因的别名)。
Recombinant human FLJ00060 protein is a genetically engineered protein derived from the FLJ00060 gene, also known as FKSG or C17orf77. This gene is located on chromosome 17q12 and encodes a conserved protein with a molecular weight of approximately 18-20 kDa. FLJ00060 contains a putative kinase domain and is classified as a member of the protein kinase superfamily, though its precise catalytic activity and biological substrates remain under investigation. Studies suggest potential roles in cellular signaling pathways, particularly in regulating cell proliferation, apoptosis, and differentiation. Its expression has been detected in various tissues, with higher levels observed in the brain, testis, and immune cells. The recombinant form is typically produced in heterologous systems like *E. coli* or mammalian cell lines (e.g., HEK293) to ensure proper post-translational modifications and functional activity. Purification methods often employ affinity tags such as His-tags for enhanced yield and stability. Current research focuses on elucidating its interaction networks, substrate specificity, and relevance to diseases like cancer, neurodegenerative disorders, and metabolic syndromes. FLJ00060's modular structure and potential regulatory functions make it a candidate for therapeutic target exploration or as a biomarker in diagnostic applications. However, comprehensive mechanistic insights and validation in *in vivo* models are still needed to establish its pathophysiological significance.
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