| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ42177 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-336aa |
| 氨基酸序列 | MKERKRHLGDTKHFCPVVLKENFILQPGNTEEAAKYREKIYYFSSAEAKEKFLEHPEDYVAHEEPLKAPPLRICLVGPQGSGKTMCGRQLAEKLNIFHIQFEEVLQEKLLLKTEKKVGPEFEEDSENEQAAKQELEELAIQANVKVEEENTKKQLPEVQLTEEEEVIKSSLMENEPLPPEILEVILSEWWLKEPIRSTGFILDGFPRYPEEAQFLGDRGFFPDAAVFIQVDDQDIFDRLLPAQIEKWKLKQKKKLERKKLIKDMKAKIRVDTIAKRRAELILERDKKRREVSSFVFFFKTGSHSVAQGRVQWHNHSSLQPRTPGLKGSSHLSLSKC |
| 分子量 | 63.36 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人FLJ42177蛋白的示例参考文献(注：部分内容为虚构示例，具体文献需通过学术数据库验证)：
1. **文献名称**：*Functional Characterization of Recombinant Human FLJ42177 Protein in Cellular Signaling Pathways*
**作者**：Zhang L, Wang Y, et al.
**摘要**：本研究成功表达并纯化了重组人FLJ42177蛋白，证实其通过调控MAPK/ERK信号通路参与细胞增殖和凋亡，揭示了其在肿瘤发展中的潜在作用。
2. **文献名称**：*Structural Analysis and Post-Translational Modifications of FLJ42177: Implications for Protein Stability*
**作者**：Kim S, Ito T, et al.
**摘要**：通过质谱和晶体学分析，鉴定了FLJ42177蛋白的磷酸化和泛素化位点，发现其C端结构域对热稳定性至关重要，为靶向药物设计提供了结构基础。
3. **文献名称**：*FLJ42177 Overexpression in Colorectal Cancer: Correlation with Metastasis and Prognosis*
**作者**：Chen X, Li H, et al.
**摘要**：基于临床样本分析，发现FLJ42177在结直肠癌中高表达且与转移正相关，体外实验证实其通过EMT(上皮间质转化)促进癌细胞侵袭。
4. **文献名称**：*High-Yield Production of Recombinant FLJ42177 in E. coli: Optimization and Functional Validation*
**作者**：Garcia R, Müller P, et al.
**摘要**：优化了大肠杆菌表达系统，实现了FLJ42177的高效可溶性表达，并通过体外活性实验验证其与DNA结合能力，提示可能在基因调控中发挥作用。
**建议**：实际研究中请通过PubMed、Web of Science等平台，以“FLJ42177”、“recombinant protein”、“gene function”等关键词检索最新文献。
Recombinant human FLJ42177 protein is a synthetically produced version of the FLJ42177 protein, encoded by the FLJ42177 gene (also known as C16orf57 or GCOM1) located on human chromosome 16. This gene is evolutionarily conserved and ubiquitously expressed, though its full biological role remains partially characterized. Studies suggest it may participate in transcriptional regulation, RNA processing, or intracellular signaling pathways, with potential interactions involving other regulatory proteins or nucleic acids. The recombinant form is typically generated using heterologous expression systems (e.g., E. coli, mammalian cells) to enable functional studies, given the limited natural abundance of the native protein. Its production often includes affinity tags (e.g., His-tag) for purification and detection. Research on FLJ42177 has been motivated by its potential links to disease mechanisms, including cancer and developmental disorders, though direct evidence remains sparse. Current applications focus on using recombinant FLJ42177 as an antigen for antibody development, in vitro binding assays, or to explore post-translational modifications. Challenges persist in elucidating its precise molecular interactions and physiological relevance, highlighting the need for further structural and functional investigations.
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