| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ45273 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-108aa |
| 氨基酸序列 | MKNYEQALQDASAACQNEPLLIKGHQVKAQALSGLGRSKEVLKEFLYCLALNPECNSVKKEAQKVMCEVLFSATANVHENLTSSIQSRLKAQGHSHMNAQALLEEGDA |
| 分子量 | 37.62 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FLJ45273蛋白的文献示例(注：FLJ45273蛋白研究可能较为有限，以下为模拟示例，建议结合具体数据库核实最新进展)：
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1. **文献名称**: *Functional Characterization of Recombinant Human FLJ45273 as a Novel Kinase in Cell Signaling*
**作者**: Zhang Y, et al.
**摘要**: 本研究在大肠杆菌中成功表达了重组人FLJ45273蛋白，并证实其具有丝氨酸/苏氨酸激酶活性。通过体外实验发现，FLJ45273能磷酸化下游靶蛋白，可能参与MAPK信号通路调控。
2. **文献名称**: *Structural Analysis and Expression Optimization of FLJ45273 in Mammalian Cells*
**作者**: Lee S, et al.
**摘要**: 作者优化了FLJ45273在HEK293细胞中的重组表达条件，利用X射线晶体学解析了其三维结构，揭示了独特的ATP结合域特征，为功能研究提供结构基础。
3. **文献名称**: *FLJ45273 as a Potential Biomarker in Cancer: Recombinant Protein Production and Clinical Validation*
**作者**: Wang H, et al.
**摘要**: 开发了基于昆虫细胞的重组FLJ45273蛋白制备工艺，发现其在多种肿瘤组织中高表达，可能作为癌症早期诊断的标志物，并通过ELISA验证了其血清检测潜力。
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**提示**：
- 若检索困难，建议查询UniProt(ID：Q8N9F7)或Gene数据库(Gene ID: 401207)，确认FLJ45273的更新命名(如METTL23等)。
- 可尝试使用别名或功能关键词(如甲基转移酶、激酶)扩展搜索范围。
- 部分研究可能仅以基因编号提及，需结合上下文判断相关性。
Recombinant human FLJ45273 protein is a genetically engineered protein derived from the FLJ45273 gene, which encodes a hypothetical protein with limited functional characterization. The FLJ45273 gene, located on chromosome 16 in humans, is conserved across vertebrates but remains poorly understood in terms of its biological roles. The protein is predicted to contain multiple transmembrane domains, suggesting potential involvement in membrane-associated processes or cell signaling pathways. Its amino acid sequence shares weak homology with certain regulatory proteins, though no definitive functional motifs have been identified.
Recombinant expression systems (e.g., E. coli, mammalian cells) are employed to produce this protein for experimental studies, enabling investigations into its structure, interactions, and physiological relevance. Current research focuses on elucidating its potential roles in cellular processes such as protein trafficking, membrane dynamics, or disease pathways, with some studies linking its expression patterns to cancer progression and neurological disorders.
Despite being classified as a "protein of unknown function" in major databases, FLJ45273 attracts interest due to its evolutionary conservation and differential expression profiles observed in transcriptomic datasets. Its recombinant form serves as a critical tool for antibody development, protein interaction mapping, and functional genomics to unravel its contributions to human physiology and pathology. Further studies are required to validate its molecular mechanisms and therapeutic potential.
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