| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FMNL1 |
| Uniprot No | O95466 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-463aa |
| 氨基酸序列 | MPLLNWVALKPSQITGTVFTELNDEKVLQELDMSDFEEQFKTKSQGPSLDLSALKSKAAQKAPSKATLIEANRAKNLAITLRKGNLGAERICQAIEAYDLQALGLDFLELLMRFLPTEYERSLITRFEREQRPMEELSEEDRFMLCFSRIPRLPERMTTLTFLGNFPDTAQLLMPQLNAIIAASMSIKSSDKLRQILEIVLAFGNYMNSSKRGAAYGFRLQSLDALLEMKSTDRKQTLLHYLVKVIAEKYPQLTGFHSDLHFLDKAGSVSLDSVLADVRSLQRGLELTQREFVRQDDCMVLKEFLRANSPTMDKLLADSKTAQEAFESVVEYFGENPKTTSPGLFFSLFSRFIKAYKKAEQEVEQWKKEAAAQEAGADTPGKGEPPAPKSPPKARRPQMDLISELKRRQQKEPLIYESDRDGAIEDIITDLRNQPYIRADTGRRSARRRPPGPPLQVTSEVSL |
| 分子量 | 78.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FMNL1蛋白的3篇参考文献及其概要：
1. **"FMNL1 Formin Homology Domain Structure and Its Role in Actin Assembly" by Smith et al. (2015)**
该研究解析了FMNL1的formin同源结构域(FH2)晶体结构，发现其通过促进肌动蛋白成核和端部加帽调控细胞骨架动力学。通过重组蛋白实验揭示了其与肌动蛋白丝的相互作用机制。
2. **"Recombinant FMNL1 Regulates T Cell Polarity and Migration via CDC42 Interaction" by Li & Chen (2018)**
文章报道重组人FMNL1通过结合小G蛋白CDC42调控T细胞极性形成，促进免疫突触处微丝聚合，从而增强T细胞迁移和抗原呈递能力。实验采用原核表达系统纯化FMNL1功能域。
3. **"Functional Characterization of FMNL1 Isoforms in Tumor Cell Invasion" by Gomez et al. (2020)**
研究对比了两种重组FMNL1剪接异构体的功能差异，发现全长异构体通过RhoA信号通路促进乳腺癌细胞侵袭，而截短型则起抑制作用，提示其在癌症转移中的潜在治疗靶点。
注：上述文献为模拟示例，实际引用需查询具体数据库确认。建议通过PubMed或Web of Science以“recombinant FMNL1”和“formins”为关键词查找最新实证研究。
Formin-like protein 1 (FMNL1) is a member of the formin family of cytoskeleton-regulating proteins, which play critical roles in actin dynamics, cell motility, and cytokinesis. FMNL1 contains conserved formin homology (FH) domains, particularly the FH2 domain, enabling its interaction with actin filaments to regulate their polymerization and organization. It is expressed in various tissues, with higher abundance in immune cells, endothelial cells, and certain cancer types, suggesting involvement in immune responses, vascular development, and tumor progression.
Functionally, FMNL1 facilitates actin-mediated processes such as cell migration, adhesion, and membrane protrusion formation (e.g., filopodia). It collaborates with Rho GTPases, notably Cdc42. to coordinate cytoskeletal remodeling in response to extracellular signals. Studies implicate FMNL1 in pathological contexts, including cancer metastasis, where its upregulation enhances invasive potential, and inflammatory diseases by modulating leukocyte trafficking.
Recombinant human FMNL1 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), retains functional domains essential for biochemical and cellular studies. It serves as a tool to investigate FMNL1's interaction partners, enzymatic activity, and role in cytoskeletal dynamics. Its applications span basic research, drug screening for FMNL1-related diseases, and elucidating mechanistic pathways in developmental and pathological processes.
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