| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLYWCH1 |
| Uniprot No | Q4VC44 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-716aa |
| 氨基酸序列 | MPLPEPSEQEGESVKAGQEPSPKPGTDVIPAAPRKPREFSKLVLLTASDQDEDGVGSKPQEVHCVLSLEMAGPATLASTLQILPVEEQGGVVQPALEMPEQKCSKLDAAAPQSLEFLRTPFGGRLLVLESFLYKQEKAVGDKVYWKCRQHAELGCRGRAITRGLRATVMRGHCHAPDEQGLEARRQREKLPSLALPEGLGEPQGPEGPGGRVEEPLEGVGPWQCPEEPEPTPGLVLSKPALEEEEAPRALSLLSLPPKKRSILGLGQARPLEFLRTCYGGSFLVHESFLYKREKAVGDKVYWTCRDHALHGCRSRAITQGQRVTVMRGHCHQPDMEGLEARRQQEKAVETLQAGQDGPGSQVDTLLRGVDSLLYRRGPGPLTLTRPRPRKRAKVEDQELPTQPEAPDEHQDMDADPGGPEFLKTPLGGSFLVYESFLYRREKAAGEKVYWTCRDQARMGCRSRAITQGRRVTVMRGHCHPPDLGGLEALRQREKRPNTAQRGSPGGPEFLKTPLGGSFLVYESFLYRREKAAGEKVYWTCRDQARMGCRSRAITQGRRVMVMRRHCHPPDLGGLEALRQREHFPNLAQWDSPDPLRPLEFLRTSLGGRFLVHESFLYRKEKAAGEKVYWMCRDQARLGCRSRAITQGHRIMVMRSHCHQPDLAGLGALRQRERLPTTAQQEDPEKIQVQLCFKTCSPESQQIYGDIKDVRLDGESQ |
| 分子量 | 106.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FLYWCH1蛋白的示例文献条目(注：部分内容为虚构示例，仅供参考，实际文献需通过学术数据库检索确认)：
---
1. **文献名称**: **"Expression and Purification of Recombinant Human FLYWCH1 Protein for Functional Studies"**
**作者**: Zhang et al. (2020)
**摘要**: 本研究描述了大肠杆菌中重组人FLYWCH1蛋白的高效表达和纯化流程，验证了其锌指结构域的完整性，并证实其在体外具有结合特定DNA序列的能力，为后续功能研究提供了材料基础。
2. **文献名称**: **"Structural Insights into FLYWCH1 Protein and Its Role in Transcriptional Regulation"**
**作者**: Lee et al. (2019)
**摘要**: 通过X射线晶体学解析了重组FLYWCH1蛋白的N端结构域，发现其通过锌指基序与靶DNA结合，并参与调控Wnt/β-catenin信号通路相关基因的转录活性。
3. **文献名称**: **"FLYWCH1 Interacts with β-Catenin to Modulate Epithelial-Mesenchymal Transition in Cancer Cells"**
**作者**: Gupta et al. (2021)
**摘要**: 利用重组FLYWCH1蛋白进行免疫共沉淀实验，发现其直接与β-catenin相互作用，抑制癌细胞的上皮-间质转化(EMT)，提示其可能作为肿瘤抑制因子发挥作用。
4. **文献名称**: **"Functional Analysis of FLYWCH1 Mutations in Developmental Disorders"**
**作者**: Müller et al. (2018)
**摘要**: 通过重组蛋白体外实验和患者突变体分析，发现FLYWCH1的结构突变会破坏其DNA结合能力，导致胚胎发育过程中的基因表达失调，与神经发育异常相关。
---
**注意**：上述文献为示例性质，实际研究中相关文献可能较少或研究方向不同。建议通过PubMed、Google Scholar等平台检索关键词 **"Recombinant human FLYWCH1"** 或 **"FLYWCH1 protein function"** 获取真实文献。
Recombinant human FLYWCH1 protein is a product of the FLYWCH1 gene, encoding a member of the FLYWCH family of transcription factors. Characterized by an N-terminal FLYWCH domain (conserved aromatic residue-rich motif) and a C-terminal zinc finger domain, FLYWCH1 functions as a transcriptional regulator. Studies suggest its involvement in modulating chromatin structure and gene expression, particularly through interactions with the ATAC complex (Ada-Two-A-containing complex), which is crucial for histone acetylation and chromatin remodeling. FLYWCH1 is implicated in diverse cellular processes, including cell proliferation, differentiation, and DNA damage response. Notably, it has been linked to Wnt/β-catenin signaling regulation, with emerging evidence pointing to its role as a tumor suppressor in certain cancers, such as colorectal cancer, where it may inhibit β-catenin-mediated oncogenic transcription. Its dysregulation has also been associated with developmental disorders and inflammatory pathways. Recombinant FLYWCH1 protein, typically produced in bacterial (e.g., E. coli) or mammalian expression systems, enables biochemical studies (e.g., DNA/protein binding assays), functional analyses, and antibody development. Purification methods often employ affinity tags (e.g., His-tag) and chromatography techniques. Current research focuses on elucidating its structural motifs, interaction networks, and therapeutic potential in diseases involving transcriptional or epigenetic dysregulation.
×
