| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ46266 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-178aa |
| 氨基酸序列 | METGPSEEPSGRKESQEMCPPGLLVFAGSSEQDANLAKQFWISASMYPPSESQLVLRRDSSQRLPVARPRRSRGSENSHSSQSFHLASNKNRDIFAEALKIQESEEKVKYLQKTRSHSVTQNEVQWHDHGSLQPQLSRIQAKTREEILQLLRKQREERISKELISLPYKPKAKEHKAE |
| 分子量 | 46.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是三篇与重组人FLJ46266蛋白相关的虚拟参考文献示例(基于典型研究场景模拟，实际文献需通过数据库验证)：
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1. **文献名称**: *Expression and functional analysis of recombinant human FLJ46266 in cancer cell lines*
**作者**: Zhang L, et al.
**摘要**: 本研究首次报道了FLJ46266蛋白的重组表达与纯化，验证其在HEK293细胞中的过表达对肿瘤细胞增殖的抑制作用，提示其可能通过调控Wnt信号通路参与癌症发展。
2. **文献名称**: *Structural characterization of FLJ46266: A putative metalloprotease domain-containing protein*
**作者**: Tanaka K, et al.
**摘要**: 通过生物信息学预测FLJ46266蛋白含锌离子结合结构域，利用大肠杆菌系统实现重组表达，并采用圆二色谱分析其二级结构，为后续酶活性研究提供基础。
3. **文献名称**: *FLJ46266 interacts with HSP90: Implications for protein stability regulation*
**作者**: Wang Y, et al.
**摘要**: 通过免疫共沉淀技术发现FLJ46266与分子伴侣HSP90存在相互作用，证明重组FLJ46266的稳定性依赖于HSP90活性，提示其在细胞应激响应中的潜在作用。
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**备注**：
- 实际研究中，FLJ46266可能已被重命名(如基于功能的基因符号，例：C1orf123或特定酶名称)，建议通过NCBI Gene或UniProt更新基因信息。
- 若文献不足，可扩大检索范围至该蛋白所属家族(如含DUF结构域蛋白)或相关通路的研究。
- 推荐数据库：PubMed、Web of Science(关键词："FLJ46266" OR "gene symbol" + "recombinant expression")。
Recombinant human FLJ46266 protein is a genetically engineered protein derived from the FLJ46266 gene, a less characterized member of the human genome initially identified through large-scale sequencing projects. The FLJ46266 gene encodes a putative protein with a molecular function yet to be fully elucidated, though bioinformatic analyses suggest potential structural motifs or domains associated with regulatory roles in cellular processes. Recombinant production of FLJ46266 typically involves heterologous expression systems, such as Escherichia coli or mammalian cell lines, enabling the study of its biochemical properties and interactions in controlled experimental settings.
Current knowledge about FLJ46266 remains limited, but its expression profile across tissues hints at possible roles in developmental or homeostatic pathways. Some studies link it to pathways involving cell adhesion, signal transduction, or metabolic regulation, though mechanistic insights are sparse. The recombinant protein serves as a critical tool for generating antibodies, conducting functional assays (e.g., protein-protein interaction screens), and exploring its potential involvement in disease contexts, such as cancer or metabolic disorders, where dysregulation of uncharacterized proteins is increasingly scrutinized.
Research on recombinant FLJ46266 is driven by the broader aim of annotating "orphan" proteins in the human proteome. Its characterization could uncover novel biomarkers or therapeutic targets. However, gaps persist in understanding its physiological relevance, post-translational modifications, or tissue-specific isoforms. Collaborative efforts integrating omics data, structural biology, and in vivo models are essential to decode its functional significance and contextualize its role in human biology and pathology.
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