| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ46111 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-197aa |
| 氨基酸序列 | MNKVELESLLEGLTDEINFLRQLYEEEIWELQSQISDTSVVLSMDSSRSLDMDSIIPEVKAQYKEIANGSWAEAEGMHQIKYEELQTLPGKHRNDLRYAKMEISEINRNISRLQAQTEGLKGQRVSLEAAIADAEQYGELAVKDANTKLSSWRPPCSGPSKTWCSSCAMEHQELMNVKLALDIKIATYRKLLEGEES |
| 分子量 | 48.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于FLJ46111蛋白的研究在公开数据库中较为有限，且可能存在基因命名更新或研究文献较少的情况，以下是基于类似蛋白研究框架构建的**假设性参考文献示例**(真实文献需进一步验证)：
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1. **文献名称**: *"Characterization of Recombinant Human FLJ46111 Protein: Structural Insights and Functional Implications"*
**作者**: Zhang Y, et al.
**摘要**: 研究通过大肠杆菌系统成功表达并纯化了重组人FLJ46111蛋白，利用圆二色谱和X射线晶体学揭示了其含有保守的磷酸酶结构域。功能实验显示该蛋白可能参与调控Wnt/β-catenin信号通路，影响肿瘤细胞迁移。
2. **文献名称**: *"FLJ46111 as a Novel Regulator of Neuronal Apoptosis: Insights from Recombinant Protein Studies"*
**作者**: Lee S, et al.
**摘要**: 通过哺乳动物细胞表达体系获得FLJ46111重组蛋白，发现其与ASK1蛋白互作并抑制JNK/p38信号通路激活，减少氧化应激诱导的神经元凋亡，提示其在中枢神经系统疾病中的潜在保护作用。
3. **文献名称**: *"Proteomic Screening Identifies FLJ46111 Interaction with DNA Repair Machinery"*
**作者**: Gupta R, et al.
**摘要**: 利用重组FLJ46111蛋白进行酵母双杂交筛选，发现其与BRCA1和RAD51存在物理相互作用，可能通过同源重组途径参与DNA损伤修复，为癌症治疗靶点开发提供新方向。
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**注意事项**：
1. 上述文献为模拟案例，实际研究中FLJ46111可能已被重新命名(如VSP36等)，建议通过UniProt (ID: Q8N5R7) 或GeneCards确认最新信息。
2. 若需真实文献，可尝试在PubMed中用关键词 "FLJ46111" 或 "VSP36" 结合 "recombinant protein" 检索，或关注其功能相似的磷酸酶家族研究。
Recombinant human FLJ46111 protein is a poorly characterized protein encoded by the FLJ46111 gene, also known as C16orf87 (chromosome 16 open reading frame 87). This protein is conserved across mammals but remains largely understudied, with limited functional or structural data available in current scientific literature. Bioinformatics analyses suggest it is a small, soluble cytoplasmic or nucleoplasmic protein, potentially involved in basic cellular processes, such as RNA metabolism, protein-protein interactions, or regulatory pathways. Its amino acid sequence contains predicted phosphorylation sites and a conserved domain of unknown function (DUF4769), which may mediate molecular recognition or enzymatic activity.
The recombinant form is typically expressed in Escherichia coli or mammalian systems using genetic engineering techniques, enabling studies to elucidate its biological roles. While no direct disease associations have been conclusively established, genomic studies occasionally link FLJ46111’s locus (16p13.3) to neurological disorders and cancer susceptibility, warranting further investigation. Current research focuses on characterizing its interactome, subcellular localization, and possible involvement in stress response or cell cycle regulation. As a recombinant tool, it serves antibody production, structural analysis, and functional screening, aiming to uncover its contribution to cellular physiology and pathology. Challenges persist due to the lack of specific biomarkers and functional redundancy with other proteins.
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