| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLYWCH2 |
| Uniprot No | Q96CP2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-140aa |
| 氨基酸序列 | MPLPEPSEQEGESVKASQEPSPKPGTEVIPAAPRKPRKFSKLVLLTASKDSTKVAGAKRKGVHCVMSLGVPGPATLAKALLQTHPEAQRAIEAAPQEPEQKRSRQDPGTDRTEDSGLAAGPPEAAGENFAPCSVAPGKSL |
| 分子量 | 15.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FLYWCH2蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**：*Functional Analysis of Recombinant Human FLYWCH2 in Transcriptional Regulation*
**作者**：Chen L, et al.
**摘要**：本研究成功表达了重组人FLYWCH2蛋白，并发现其通过结合β-catenin抑制Wnt信号通路，在结直肠癌细胞中调控基因转录，为肿瘤治疗提供潜在靶点。
2. **文献名称**：*Expression and Purification of FLYWCH2: A Zinc Finger Protein Involved in DNA Repair*
**作者**：Kim S, et al.
**摘要**：文章优化了重组人FLYWCH2蛋白在大肠杆菌中的表达条件，纯化后验证其结合DNA损伤位点的能力，揭示了其在修复双链断裂中的作用机制。
3. **文献名称**：*FLYWCH2 as a Tumor Suppressor: Role in Epithelial-Mesenchymal Transition*
**作者**：Wang Y, et al.
**摘要**：通过重组FLYWCH2蛋白的功能实验，发现其通过抑制Snail蛋白活性阻断EMT过程，降低乳腺癌细胞迁移能力，提示其抗转移潜力。
4. **文献名称**：*Structural Characterization of FLYWCH2 and Its Interaction with Chromatin Modifiers*
**作者**：Garcia R, et al.
**摘要**：利用重组蛋白结晶技术解析FLYWCH2结构，发现其锌指结构域与组蛋白去乙酰化酶(HDAC)结合，参与表观遗传调控。
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**注意**：以上文献为模拟示例，实际研究中FLYWCH2相关研究较少，建议核实蛋白名称拼写或拓展检索范围(如FLYWCH1或其他转录因子家族)。如需真实文献，可通过PubMed或Web of Science以“FLYWCH2”或“FLYWCH protein”为关键词检索。
The FLYWCH2 protein, a member of the FLYWCH family, is a transcription factor characterized by its conserved FLYWCH domain, which facilitates DNA-binding and protein-protein interactions. Initially identified through homology studies, human FLYWCH2 (hFLYWCH2) is implicated in regulating gene expression, particularly in pathways involving cell proliferation, differentiation, and tumor suppression. Its role in modulating Wnt/β-catenin signaling has drawn attention, as dysregulation of this pathway is linked to cancers and developmental disorders. Recombinant human FLYWCH2 (rhFLYWCH2) is typically produced via bacterial or mammalian expression systems, enabling studies on its structural and functional properties. Researchers utilize rhFLYWCH2 to explore its interaction with DNA or partner proteins, such as β-catenin, and to assess its potential as a therapeutic target. Studies suggest FLYWCH2 may act as a tumor suppressor by inhibiting oncogenic transcriptional activity, though its mechanisms remain under investigation. Its recombinant form is also valuable for generating antibodies, diagnostic tools, or high-throughput assays. Despite progress, the full spectrum of FLYWCH2’s biological roles and clinical relevance requires further elucidation, particularly in tissue-specific contexts and disease models. Current research focuses on unraveling its post-translational modifications, subcellular localization, and cross-talk with other signaling networks.
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