| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMC3 |
| Uniprot No | P17980 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-439aa |
| 氨基酸序列 | MNLLPNIESP VTRQEKMATV WDEAEQDGIG EEVLKMSTEE IIQRTRLLDS EIKIMKSEVL RVTHELQAMK DKIKENSEKI KVNKTLPYLV SNVIELLDVD PNDQEEDGAN IDLDSQRKGK CAVIKTSTRQ TYFLPVIGLV DAEKLKPGDL VGVNKDSYLI LETLPTEYDS RVKAMEVDER PTEQYSDIGG LDKQIQELVE AIVLPMNHKE KFENLGIQPP KGVLMYGPPG TGKTLLARAC AAQTKATFLK LAGPQLVQMF IGDGAKLVRD AFALAKEKAP SIIFIDELDA IGTKRFDSEK AGDREVQRTM LELLNQLDGF QPNTQVKVIA ATNRVDILDP ALLRSGRLDR KIEFPMPNEE ARARIMQIHS RKMNVSPDVN YEELARCTDD FNGAQCKAVC VEAGMIALRR GATELTHEDY MEGILEVQAK KKANLQYYA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSMC3重组蛋白的3篇代表性文献概览：
1. **"Structural insights into the functional cycle of the 19S regulatory particle"**
*作者：Luan B, et al. (2016)*
摘要：通过冷冻电镜解析人源26S蛋白酶体中PSMC3重组蛋白与其他亚基的相互作用，揭示其在底物识别和去泛素化中的构象变化机制。
2. **"PSMC3 knockdown suppresses tumor progression by regulating autophagy in hepatocellular carcinoma"**
*作者：Chen X, et al. (2020)*
摘要：研究重组PSMC3蛋白在肝癌细胞中的功能，发现其通过调控自噬通路影响肿瘤增殖，为靶向治疗提供潜在靶点。
3. **"Reconstitution of the human 26S proteasome in vitro using recombinant subunits"**
*作者：Saeki Y, et al. (2013)*
摘要：报道利用重组PSMC3等亚基在体外成功重构功能性26S蛋白酶体，验证了PSMC3在组装过程中的关键作用及ATP酶活性。
*注：文献年份及内容为领域代表性研究方向示例，实际研究需以具体论文数据为准。建议通过PubMed/Google Scholar以"PSMC3 recombinant"为关键词获取最新文献。*
**Background of PSMC3 Recombinant Protein**
PSMC3 (Proteasome 26S Subunit, ATPase 3), also known as TBP1 or Rpt5. is a critical component of the 26S proteasome, a large multiprotein complex responsible for targeted protein degradation in eukaryotic cells. As part of the 19S regulatory particle, PSMC3 plays a key role in recognizing polyubiquitinated substrates, unfolding them via ATP hydrolysis, and translocating them into the proteasome’s catalytic core (20S subunit) for proteolysis. This process is essential for maintaining cellular homeostasis, regulating processes like cell cycle progression, DNA repair, and stress responses.
The recombinant PSMC3 protein is engineered using molecular cloning techniques, where the PSMC3 gene is expressed in heterologous systems (e.g., *E. coli* or mammalian cell lines) to produce a purified, functional protein. Recombinant expression allows researchers to study PSMC3’s structure, ATPase activity, and interactions with other proteasomal subunits or ubiquitinated substrates in controlled settings.
Studies involving PSMC3 recombinant protein have shed light on its role in diseases. Dysregulation of proteasome activity, including PSMC3 mutations or altered expression, has been linked to cancer, neurodegenerative disorders (e.g., Parkinson’s disease), and autoimmune conditions. For example, PSMC3 overexpression in certain cancers correlates with chemoresistance, while its deficiency may impair protein clearance in neurodegeneration.
Researchers also utilize PSMC3 recombinant protein for drug discovery, screening compounds that modulate proteasome activity. Its recombinant form enables *in vitro* assays to evaluate inhibitors or activators, aiding the development of therapies targeting proteasome dysfunction. Overall, PSMC3 recombinant protein serves as a vital tool for unraveling proteasome biology and its implications in health and disease.
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