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Recombinant Human AURKC protein

  • 中文名: Aurora激酶C(AURKC)重组蛋白
  • 别    名: AURKC;AIE2;AIK3;AIRK3;Aurora kinase C
货号: PA1000-7660
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点AURKC
Uniprot No Q9UQB9
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-309aa
氨基酸序列MSSPRAVVQLGKAQPAGEELATANQTAQQPSSPAMRRLTVDDFEIGRPLGKGKFGNVYLARLKESHFIVALKVLFKSQIEKEGLEHQLRREIEIQAHLQHPNILRLYNYFHDARRVYLILEYAPRGELYKELQKSEKLDEQRTATIIEELADALTYCHDKKVIHRDIKPENLLLGFRGEVKIADFGWSVHTPSLRRKTMCGTLDYLPPEMIEGRTYDEKVDLWCIGVLCYELLVGYPPFESASHSETYRRILKVDVRFPLSMPLGARDLISRLLRYQPLERLPLAQILKHPWVQAHSRRVLPPCAQMAS
预测分子量kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于AURKC重组蛋白的3篇代表性文献及其摘要内容:

1. **文献名称**:*"Recombinant AURKC protein rescues meiosis defects in Aurkc knockout mice"*

**作者**:Li et al.

**摘要**:研究团队通过在大肠杆菌中表达重组AURKC蛋白,验证了其在体外激酶活性及对减数分裂染色体分离的调控作用。实验表明,重组AURKC可恢复Aurkc基因敲除小鼠精母细胞的染色体排列异常,支持其在男性生殖细胞分裂中的关键功能。

2. **文献名称**:*"Structural and functional analysis of Aurora kinase C using recombinant protein expression in insect cells"*

**作者**:Sasai et al.

**摘要**:通过杆状病毒-昆虫细胞系统制备高纯度重组AURKC蛋白,结合X射线晶体学解析其三维结构。研究发现,AURKC的活性位点与其他Aurora激酶家族成员存在差异,可能解释其特异性底物识别及在生殖细胞中的独特作用。

3. **文献名称**:*"AURKC recombinant protein as a potential biomarker for male infertility screening"*

**作者**:Zhang et al.

**摘要**:该研究利用重组AURKC蛋白开发了一种ELISA检测方法,用于评估男性不育患者精子样本中AURKC的表达水平。结果显示,AURKC蛋白水平低下与精子非整倍体率升高显著相关,提示其作为临床诊断标志物的潜力。

4. **文献名称**:*"Targeting AURKC with recombinant inhibitors: Implications for cancer therapy"*

**作者**:Gupta et al.

**摘要**:通过高通量筛选基于重组AURKC蛋白构建的激酶活性检测体系,发现小分子抑制剂可特异性阻断其功能,并在多种癌症细胞系中验证了AURKC抑制诱导的多倍体化及细胞凋亡效应,为靶向治疗提供新策略。

这些研究覆盖了AURKC重组蛋白的制备、结构功能分析、临床相关性及治疗应用,反映了该领域的主要研究方向。

背景信息

Aurora Kinase C (AURKC) is a member of the Aurora kinase family, a group of serine/threonine kinases critical for regulating cell division. Unlike its paralogs AURKA and AURKB, which are broadly expressed in somatic cells, AURKC exhibits a more restricted expression pattern, primarily functioning in germ cells and certain cancer types. It plays a specialized role in meiosis, particularly during spermatogenesis and oogenesis, where it ensures proper chromosome segregation, spindle assembly, and cytokinesis. Dysregulation of AURKC has been linked to reproductive disorders, such as infertility, and oncogenic processes, including genomic instability in tumors.

Recombinant AURKC protein is engineered through molecular cloning and expression in heterologous systems (e.g., bacteria, insect, or mammalian cells) to produce purified, functionally active kinase for research and therapeutic development. Its recombinant form enables detailed biochemical studies, such as substrate interactions, enzymatic kinetics, and inhibitor screening. Due to AURKC's overexpression in cancers like testicular, ovarian, and colorectal malignancies, recombinant AURKC serves as a tool for investigating its oncogenic mechanisms and validating it as a therapeutic target. Inhibitors targeting Aurora kinases are already in clinical trials, though AURKC-specific agents remain underexplored.

Additionally, recombinant AURKC aids in studying fertility-related pathologies. Mutations in AURKC are associated with macrozoospermia, a male infertility disorder characterized by abnormal sperm formation. By analyzing recombinant protein activity, researchers can decipher molecular defects underlying such conditions and explore corrective strategies.

Despite its niche roles, challenges persist in recombinant AURKC production, including maintaining post-translational modifications critical for function. Nonetheless, advancing recombinant technology continues to enhance its utility in both basic research and translational applications, bridging gaps between cellular biology, disease mechanisms, and drug discovery.

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