| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMC4 |
| Uniprot No | P43686 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-418aa |
| 氨基酸序列 | MEEIGILVEK AQDEIPALSV SRPQTGLSFL GPEPEDLEDL YSRYKKLQQE LEFLEVQEEY IKDEQKNLKK EFLHAQEEVK RIQSIPLVIG QFLEAVDQNT AIVGSTTGSN YYVRILSTID RELLKPNASV ALHKHSNALV DVLPPEADSS IMMLTSDQKP DVMYADIGGM DIQKQEVREA VELPLTHFEL YKQIGIDPPR GVLMYGPPGC GKTMLAKAVA HHTTAAFIRV VGSEFVQKYL GEGPRMVRDV FRLAKENAPA IIFIDEIDAI ATKRFDAQTG ADREVQRILL ELLNQMDGFD QNVNVKVIMA TNRADTLDPA LLRPGRLDRK IEFPLPDRRQ KRLIFSTITS KMNLSEEVDL EDYVARPDKI SGADINSICQ ESGMLAVREN RYIVLAKDFE KAYKTVIKKD EQEHEFYK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSMC4重组蛋白的参考文献示例(注：以下内容为示例性概括，具体文献需通过学术数据库检索确认)：
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1. **文献名称**：*"Recombinant expression and functional characterization of human PSMC4 in the 26S proteasome"*
**作者**：Smith J, et al.
**摘要**：本研究成功在大肠杆菌中表达了重组人源PSMC4蛋白，并验证其作为26S蛋白酶体ATP酶亚基的功能，证实其参与底物识别及ATP依赖的蛋白降解过程。
2. **文献名称**：*"Structural insights into PSMC4 conformational dynamics using cryo-EM"*
**作者**：Chen L, et al.
**摘要**：通过冷冻电镜解析重组PSMC4蛋白的三维结构，揭示其在蛋白酶体组装中的构象变化及与其他亚基(如PSMC1/PSMC2)的互作机制。
3. **文献名称**：*"PSMC4 knockdown impairs proteasome activity and induces cellular apoptosis via recombinant protein complementation assays"*
**作者**：Wang Y, et al.
**摘要**：利用重组PSMC4蛋白回补实验，证明PSMC4缺失导致蛋白酶体活性下降，并通过线粒体途径触发细胞凋亡，提示其肿瘤治疗潜在靶点价值。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“PSMC4 recombinant”或“PSMC4 proteasome”。
PSMC4 (Proteasome 26S Subunit, ATPase 4) is a critical component of the 26S proteasome, a multiprotein complex responsible for ubiquitin-dependent protein degradation in eukaryotic cells. As part of the AAA-ATPase family, PSMC4 contributes to the regulatory particle (PA700/19S) of the proteasome, which recognizes polyubiquitinated substrates, unfolds them via ATP hydrolysis, and translocates them into the catalytic core (20S) for proteolysis. This process is essential for maintaining cellular homeostasis, regulating cell cycle progression, stress responses, and degrading misfolded proteins.
Recombinant PSMC4 protein is engineered through genetic cloning and expression systems (e.g., E. coli, mammalian cells) to produce purified, functional forms for biochemical and biomedical research. Studies utilize recombinant PSMC4 to investigate proteasome assembly, ATPase activity, and substrate processing mechanisms. Its role in diseases, particularly cancers and neurodegenerative disorders linked to proteasomal dysfunction, has spurred interest in developing inhibitors targeting PSMC4-containing complexes for therapeutic applications.
Structurally, PSMC4 contains conserved Walker A/B motifs critical for ATP binding and hydrolysis. Recombinant variants often include affinity tags (e.g., His-tag) to facilitate purification and interaction studies. Researchers employ these proteins in pull-down assays, structural analyses (e.g., cryo-EM), and drug screening platforms. Recent work also explores PSMC4's non-proteasomal roles, such as transcriptional regulation, highlighting its multifaceted cellular importance. Quality-controlled recombinant PSMC4 remains indispensable for dissecting proteasome biology and advancing targeted therapies.
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