| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GMCL1L |
| Uniprot No | Q8NEA9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-526aa |
| 氨基酸序列 | MGSSSSRVLGQPRRALAQQEQGARARGSARRPDTGDDAASYGFCYCPGSHKRKRSSGACRYCDPDSHREEHEEEGDKQQPLLNTPARKKLRSTSKYIYQTLFLNGENSDIKICALGEEWRLHKIYLCQSGYFSSMFSGSWKESSMNIIELEIPDQNIDVDALQVAFGSLYRDDVLIKPSRVVAILAAACMLQLDGLIQQCGETMKETINVKTVCGYYTSVEIYGLDSVKKKCLEWLLNNLMTHQNVKLFKELGINVMKQLIGSSNLFVMQVEMDVYTTLKKWMFLQLVPSWNGSLKQLLTETDVWFSKQRKDFEGMAFLETEPGKPFVSVFRHLRLQYIISDLASARIIEQDGIVPSEWLSSVYKQQWFAMLRAEQDHEVGPQEINKEDLEGSSMRCGRKLAKDGEYYWCWTGFNFGFDLLVIYTNGYIIFKRNTLNQPCSGSVSLRPRRSIAFRLRLASFDSSGKLVCSRTTGYQILILKKDQEQVVMNLDSRFLTFPLYICCNFLYISPEKGIENNRHPEDPEN |
| 分子量 | 86.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GM-CSF/L1蛋白的假设性参考文献示例（注：由于该名称可能不明确，实际文献可能需要具体名称核实）：
---
1. **文献名称**: *Expression and Functional Analysis of Recombinant Human GM-CSF/L1 Fusion Protein in Hematopoiesis*
**作者**: Wang X, et al.
**摘要**: 研究报道了重组人GM-CSF/L1融合蛋白的构建及功能，发现其通过激活JAK/STAT通路显著促进骨髓前体细胞增殖，并在体外实验中显示出比天然GM-CSF更强的稳定性。
2. **文献名称**: *GM-CSF-L1 as a Novel Adjuvant in Cancer Immunotherapy: Preclinical Evaluation*
**作者**: Johnson R, et al.
**摘要**: 该研究评估了重组GM-CSF-L1蛋白作为肿瘤疫苗佐剂的潜力，证明其能增强抗原呈递细胞（APCs）的成熟和T细胞抗肿瘤反应，显著抑制小鼠黑色素瘤模型中的肿瘤生长。
3. **文献名称**: *Structural Characterization and In Vivo Efficacy of a Recombinant GM-CSF/L1 Chimeric Cytokine*
**作者**: Chen L, et al.
**摘要**: 通过X射线晶体学解析了GM-CSF/L1的融合结构，揭示L1结构域延长了GM-CSF的半衰期；动物实验表明其在急性辐射损伤模型中加速造血恢复。
4. **文献名称**: *GM-CSF/L1 Fusion Protein Modulates Macrophage Polarization in Chronic Inflammation*
**作者**: Gupta S, et al.
**摘要**: 研究表明，GM-CSF/L1重组蛋白通过调节巨噬细胞向抗炎表型（M2）极化，缓解类风湿性关节炎小鼠模型的关节炎症，提出其在自身免疫疾病中的治疗潜力。
---
**注意**：上述文献为示例性内容，实际研究可能使用不同的蛋白命名或研究方向。建议结合具体需求核实“GM-CSF/L1”的准确性（如是否为GM-CSF与IL-1或其他因子的融合体），或调整检索关键词以匹配真实文献。
**Background of Recombinant Human GMCL1L Protein**
The germ cell-less-like protein 1-like (GMCL1L), also known as CRR4L, is a nuclear-enriched protein belonging to the germ cell-less (GCL) family. It shares structural homology with GCL, characterized by a conserved BTB/POZ domain involved in protein-protein interactions and a BACK domain critical for scaffolding. GMCL1L is implicated in regulating cell cycle progression, apoptosis, and transcriptional control, potentially interacting with MYC/MAX networks to modulate gene expression.
Recombinant human GMCL1L protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells*), enabling large-scale production for functional studies. Its purification often involves affinity chromatography to ensure high purity and bioactivity. Research focuses on deciphering GMCL1L’s role in cellular homeostasis, particularly its influence on chromatin remodeling, DNA repair, and mitotic regulation. Dysregulation of GMCL1L has been linked to carcinogenesis, neurodevelopmental disorders, and infertility, making it a potential biomarker or therapeutic target.
Current studies leverage recombinant GMCL1L to explore its biochemical interactions, post-translational modifications, and tissue-specific functions, aiming to unravel its physiological and pathological significance in human health.
×
