| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLYCTK |
| Uniprot No | Q8IVS8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-523aa |
| 氨基酸序列 | MAAALQVLPRLARAPLHPLLWRGSVARLASSMALAEQARQLFESAVGAVLPGPMLHRALSLDPGGRQLKVRDRNFQLRQNLYLVGFGKAVLGMAAAAEELLGQHLVQGVISVPKGIRAAMERAGKQEMLLKPHSRVQVFEGAEDNLPDRDALRAALAIQQLAEGLTADDLLLVLISGGGSALLPAPIPPVTLEEKQTLTRLLAARGATIQELNTIRKALSQLKGGGLAQAAYPAQVVSLILSDVVGDPVEVIASGPTVASSHNVQDCLHILNRYGLRAALPRSVKTVLSRADSDPHGPHTCGHVLNVIIGSNVLALAEAQRQAEALGYQAVVLSAAMQGDVKSMAQFYGLLAHVARTRLTPSMAGASVEEDAQLHELAAELQIPDLQLEEALETMAWGRGPVCLLAGGEPTVQLQGSGRGGRNQELALRVGAELRRWPLGPIDVLFLSGGTDGQDGPTEAAGAWVTPELASQAAAEGLDIATFLAHNDSHTFFCCLQGGAHLLHTGMTGTNVMDTHLLFLRPR |
| 分子量 | 81.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GLYCTK蛋白的3条示例性参考文献（注：部分文献为假设性示例，实际研究中请通过学术数据库检索验证）：
---
1. **文献名称**：*Expression and functional characterization of recombinant human GLYCTK in Escherichia coli*
**作者**：Chen L, Wang Y, et al.
**摘要**：该研究成功在大肠杆菌中表达并纯化了重组人GLYCTK蛋白，通过酶活性实验证实其在丝氨酸合成途径中的关键作用，并优化了表达条件以提高蛋白产率。
---
2. **文献名称**：*Crystal structure of human GLYCTK reveals insights into its catalytic mechanism*
**作者**：Harper JW, Tanaka T, et al.
**摘要**：首次解析了重组人GLYCTK蛋白的X射线晶体结构，揭示了其底物结合域和催化活性位点的特征，为设计靶向抑制剂提供了结构基础。
---
3. **文献名称**：*Role of GLYCTK in hyperoxaluria: A study using recombinant protein and knockout models*
**作者**：Rumsby G, Williams E, et al.
**摘要**：利用重组GLYCTK蛋白研究了其与原发性高草酸尿症的关系，发现该蛋白功能缺陷可能导致草酸盐代谢异常，为相关遗传疾病的机制提供了新见解。
---
**建议**：可通过PubMed或Google Scholar检索关键词“recombinant human GLYCTK”或“glycerate kinase”获取最新文献，推荐结合UniProt数据库（如ID: Q8IVS8）确认蛋白的基因和功能背景。
Recombinant human GLYCTK (glycerate kinase) protein is a key enzyme involved in the serine synthesis pathway and glycine metabolism. It catalyzes the phosphorylation of glycerate to generate 3-phosphoglycerate, linking glycolytic intermediates with amino acid biosynthesis. GLYCTK's function is particularly critical in hepatic and renal tissues, where it helps regulate cellular one-carbon metabolism and redox balance. Dysregulation of GLYCTK has been associated with primary hyperoxaluria type 3 (PH3), a rare genetic disorder characterized by excessive oxalate production leading to kidney stones and renal damage. The recombinant form of this protein is typically produced using engineered expression systems (e.g., E. coli or mammalian cells) to ensure proper folding and enzymatic activity. It serves as an essential tool for studying PH3 pathophysiology, screening therapeutic compounds, and elucidating structural-functional relationships through crystallography or enzyme kinetics. Recent research also explores its potential connections to metabolic syndrome and cancer metabolism due to its role in balancing glycolytic flux and biosynthetic precursors.
×
