| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNB2L1 |
| Uniprot No | P63244 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317aa |
| 氨基酸序列 | MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR |
| 分子量 | 60.72 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GNB2L1（RACK1）蛋白的3篇参考文献，包含标题、作者及简要摘要内容：
1. **标题**：*RACK1, a receptor for activated protein kinase C, interacts with β‐integrin subunits*
**作者**：Buensuceso CS 等
**摘要**：该研究报道了重组人GNB2L1（RACK1）通过与β-integrin的相互作用调节细胞黏附和迁移，揭示了其在细胞外基质信号传导中的调控机制。
2. **标题**：*Structural and functional analysis of the human RACK1: A platform for coordinating diverse cellular signaling pathways*
**作者**：Adams DR 等
**摘要**：通过X射线晶体学解析了重组RACK1蛋白的三维结构，阐明其作为支架蛋白在整合PKC、Src激酶等信号通路中的分子基础。
3. **标题**：*RACK1 promotes hepatocellular carcinoma progression through activating PI3K/AKT/mTOR signaling pathway*
**作者**：Li Y 等
**摘要**：研究发现，重组GNB2L1蛋白在肝癌细胞中过表达并通过激活PI3K/AKT/mTOR通路促进肿瘤增殖和转移，提示其作为潜在治疗靶点。
若需更多文献或具体研究方向，可进一步补充说明。
Recombinant human GNB2L1 protein, also known as RACK1 (Receptor for Activated C Kinase 1), is a multifaceted scaffold protein involved in diverse cellular processes. Encoded by the GNB2L1 gene, it belongs to the WD40 repeat protein family, characterized by its seven-bladed β-propeller structure that facilitates interactions with numerous signaling molecules.
RACK1 plays a pivotal role in coordinating signal transduction pathways, including those mediated by protein kinase C (PKC), MAPK, and JNK, thereby regulating cell growth, differentiation, and migration. It acts as a molecular adaptor, bridging receptors, kinases, and other effectors to modulate processes like translation, inflammation, and stress responses. Studies highlight its involvement in neurodevelopment, synaptic plasticity, and immune regulation, with implications in neurological disorders, cancer, and cardiovascular diseases.
As a recombinant protein, GNB2L1 is commonly produced in bacterial or eukaryotic expression systems for research applications. Its structural and functional versatility makes it valuable for studying protein-protein interactions, signaling mechanisms, and drug target discovery. Dysregulation of RACK1 has been linked to tumor progression, viral pathogenesis, and psychiatric conditions, driving interest in its therapeutic potential. Recombinant forms enable biochemical assays, structural studies, and exploration of its roles in disease models, aiding the development of targeted therapies.
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