| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GMEB2 |
| Uniprot No | Q9UKD1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-530aa |
| 氨基酸序列 | MATPDVSVHMEEVVVVTTPDTAVDGSGVEGVKTVLVTTNLAPHGGDLTEDNMETENAAAAAAAAFTASSQLKEAVLVKMAEEGENLEAEIVYPITCGDSRANLIWRKFVCPGINVKCVQYDEHVISPKEFVHLAGKSTLKDWKRAIRMNGIMLRKIMDSGELDFYQHDKVCSNTCRSTKIDLSGARVSLSSPTSAEYIPLTPAAADVNGSPATITIETCEDPGDWTAAIGDDTFTFWRGLKDAGLLDEVIQEFHQELVETMRGLQQRVQDPPLQLRDAVLLNNIVQNFGMLDLVKKVLASHKCQMDRSREQYARDLAALEQQCDEHRRRAKELKHKSQHLSNVLMTLTPVSLPPPVKRPRLARATSGPAAMASQVLTQSAQLALGPGVPVPQLTSVPLGKVVSTLPSTVLGKGSLQAPPASSPASPLLGGYTVLASSGSTYPSTVEIHPDASSLTVLSTAAVQDGSTVFKVVSPLQLLTLPGLGPTLQNVAQASPGSSTIVTVPAGAAPGPEEHTATIEVAAMAEDHERK |
| 分子量 | 84.04 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GMEB2蛋白的3篇参考文献及其摘要内容概括：
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1. **标题**：*Characterization of recombinant human GMEB2 and its interaction with the glucocorticoid receptor*
**作者**：Smith J, Brown KE, et al.
**摘要**：研究报道了重组人GMEB2蛋白的体外表达与纯化方法，并证实其通过结合糖皮质激素受体（GR）的调控元件，增强GR介导的转录激活活性，提示其在激素信号通路中的关键作用。
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2. **标题**：*GMEB2 modulates cell proliferation and apoptosis in cancer cells via p53 signaling*
**作者**：Lee S, Kim M
**摘要**：通过重组人GMEB2蛋白的功能实验，发现其通过调控p53依赖的基因表达网络影响癌细胞周期停滞和凋亡，揭示了GMEB2在肿瘤生物学中的潜在治疗靶点价值。
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3. **标题**：*Structural analysis of recombinant GMEB2 reveals a DNA-binding dependent mechanism*
**作者**：Zhang L, Zhou Y, et al.
**摘要**：利用X射线晶体学解析重组人GMEB2蛋白结构，发现其通过特定的α-螺旋结构域结合DNA，并依赖此结构实现与其他转录因子的协同调控，阐明其分子作用机制。
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**备注**：上述文献为示例，实际研究中建议通过PubMed或Web of Science以关键词“recombinant GMEB2”、“GMEB2 function”检索最新论文。部分研究可能聚焦于GMEB家族与病毒（如乙型肝炎病毒复制调控）或干细胞分化的关联。
Recombinant human GMEB2 (Glucocorticoid Modulatory Element-Binding Protein 2) is a protein encoded by the *GMEB2* gene, which belongs to the GMEB family. It plays a regulatory role in transcriptional processes, particularly in modulating glucocorticoid receptor (GR)-mediated gene expression. GMEB2 interacts with GMEB1 to form a heterodimeric complex, enabling DNA binding to specific glucocorticoid modulatory elements (GMEs) and influencing transcriptional activation or repression. Beyond glucocorticoid signaling, GMEB2 is implicated in diverse cellular functions, including apoptosis regulation, viral defense mechanisms, and cell cycle control. Its structural features, such as the KIST (K homology domain, Immunoglobulin-like fold, and STYK domain) motif, facilitate interactions with DNA and other proteins, underscoring its versatility in cellular processes.
Recombinant GMEB2 is typically produced using expression systems like *E. coli* or mammalian cells, allowing researchers to study its biochemical properties and mechanistic roles. It has been utilized in studies exploring its interaction with viral proteins, such as parvovirus replication, and its involvement in diseases like cancer, where dysregulation of GMEB2 may affect apoptosis or oncogenic pathways. Additionally, GMEB2's role in immune responses and stress signaling highlights its potential as a therapeutic target. The recombinant form enables structural analysis, functional assays (e.g., ELISA, co-immunoprecipitation), and drug discovery efforts. Despite progress, its full regulatory network and context-dependent functions in health and disease require further elucidation.
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