| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GMEB1 |
| Uniprot No | Q9Y692 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-563aa |
| 氨基酸序列 | MANAEVSVPVGDVVVVPTEGNEGENPEDTKTQVILQLQPVQQGIYEAGSENNTAVVAVETHTIHKIEEGIDTGTIEANEDMEIAYPITCGESKAILLWKKFVCPGINVKCVKFNDQLISPKHFVHLAGKSTLKDWKRAIRLGGIMLRKMMDSGQIDFYQHDKVCSNTCRSTKFDLLISSARAPVPGQQTSVVQTPTSADGSITQIAISEESMEEAGLEWNSALTAAVTMATEEGVKKDSEEISEDTLMFWKGIADVGLMEEVVCNIQKEIEELLRGVQQRLIQAPFQVTDAAVLNNVAHTFGLMDTVKKVLDNRRNQVEQGEEQFLYTLTDLERQLEEQKKQGQDHRLKSQTVQNVVLMPVSTPKPPKRPRLQRPASTTVLSPSPPVQQPQFTVISPITITPVGQSFSMGNIPVATLSQGSSPVTVHTLPSGPQLFRYATVVSSAKSSSPDTVTIHPSSSLALLSSTAMQDGSTLGNMTTMVSPVELVAMESGLTSAIQAVESTSEDGQTIIEIDPAPDPEAEDTEGKAVILETELRTEEKVVAEMEEHQHQVHNVEIVVLED |
| 分子量 | 87.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GMEB1蛋白的3篇参考文献的简要概括：
1. **文献名称**：*The minute virus of mice nonstructural protein NS1 interacts with the glucocorticoid modulatory element binding protein GMEB1*
**作者**：Lorson, C. et al.
**摘要**：该研究揭示了GMEB1蛋白与微小病毒非结构蛋白NS1的相互作用，证明其作为宿主因子在病毒复制中发挥作用，可能通过调节DNA结合活性促进病毒复制。
2. **文献名称**：*Identification of a Nuclear Localization Signal in the GMEB1 Protein Required for Adeno-associated Virus Type 2 Infection*
**作者**：Yoto, Y. et al.
**摘要**：研究鉴定了GMEB1的核定位信号（NLS），证明其介导腺相关病毒（AAV）的核内运输，并证实GMEB1在病毒基因组进入宿主细胞核过程中起关键作用。
3. **文献名称**：*GMEB1 promotes the activation of the p53 tumor suppressor pathway by enhancing the binding of p300 to p53*
**作者**：Jordan, M. et al.
**摘要**：本文发现GMEB1通过与p300/CBP组蛋白乙酰转移酶的相互作用，增强p53的转录活性，从而参与p53依赖性细胞凋亡和肿瘤抑制调控。
上述文献均涉及GMEB1在不同生理或病理过程中的分子机制研究，涵盖病毒复制和肿瘤调控等领域。建议通过PubMed或Web of Science搜索标题验证具体信息。
**Background of Recombinant Human GMEB1 Protein**
Glucocorticoid modulatory element-binding protein 1 (GMEB1) is a cellular protein involved in regulating gene expression by binding to glucocorticoid modulatory elements (GMEs) in DNA. It interacts with the glucocorticoid receptor (GR) signaling pathway, influencing transcriptional activation or repression in response to stress, immune responses, and metabolic processes. GMEB1 also participates in viral defense mechanisms, notably by inhibiting the double-stranded RNA-dependent protein kinase (PKR), which modulates antiviral responses and apoptosis.
Recombinant human GMEB1 protein is engineered using *in vitro* expression systems (e.g., *E. coli* or mammalian cells) to produce purified, biologically active forms of the protein for research. Its recombinant production enables studies on its structure, DNA/RNA-binding properties (via its KH domain), and interactions with partners like PARP1 or p160 coactivators. Researchers utilize recombinant GMEB1 to explore its roles in stress responses, hormone signaling, and innate immunity, as well as its implications in diseases such as cancer and autoimmune disorders. Its versatility in modulating transcription and cellular survival pathways underscores its importance in both basic and therapeutic research.
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