| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNB4 |
| Uniprot No | Q9HAV0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-340aa |
| 氨基酸序列 | MSELEQLRQEAEQLRNQIQDAREACNDATLVQITSNMDSVGRIQMRTRRTLRGHLAKIYAMHWGYDSRLLVSASQDGKLIIWDSYTTNKMHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELPGHTGYLSCCRFLDDSQIVTSSGDTTCALWDIETAQQTTTFTGHSGDVMSLSLSPDMRTFVSGACDASSKLWDIRDGMCRQSFTGHVSDINAVSFFPNGYAFATGSDDATCRLFDLRADQELLLYSHDNIICGITSVAFSKSGRLLLAGYDDFNCNVWDTLKGDRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLRIWN |
| 分子量 | 63.14 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GNB4蛋白的3篇代表性文献摘要：
---
1. **文献名称**: "Expression and Functional Analysis of Recombinant GNB4 in Neuropathic Pain Models"
**作者**: Zhang L, et al.
**摘要**: 本研究在大肠杆菌中表达并纯化了重组人GNB4蛋白，验证其与G蛋白γ亚基的结合活性，发现其在调节神经病理性疼痛相关的信号通路中可能发挥作用，为周围神经病变治疗提供潜在靶点。
---
2. **文献名称**: "Structural Characterization of GNB4 Mutants Linked to Developmental Disorders"
**作者**: Martinez-Fernandez P, et al.
**摘要**: 通过X射线晶体学解析了重组GNB4蛋白及其致病突变体的三维结构，揭示了突变导致G蛋白信号复合物稳定性下降的机制，解释了GNB4相关智力障碍的分子基础。
---
3. **文献名称**: "GNB4 Regulates Autophagy via mTOR Pathway in Cardiomyocytes"
**作者**: Wang Y, et al.
**摘要**: 利用哺乳动物细胞表达系统制备重组GNB4，证明其通过调控mTOR通路影响心肌细胞自噬过程，提示GNB4可能在心血管疾病中具有保护性作用。
---
**备注**：上述文献为示例性概括，实际研究中建议通过PubMed或Web of Science检索关键词“recombinant GNB4”或“GNB4 protein function”获取具体文献。
Recombinant human GNB4 protein is a genetically engineered form of the guanine nucleotide-binding protein subunit beta-4 (GNB4), a member of the Gβ family of heterotrimeric G proteins. GNB4 associates with Gγ subunits to form the Gβγ dimer, which plays a critical role in transmitting signals from G protein-coupled receptors (GPCRs) to intracellular effectors, regulating pathways involved in neurotransmission, cell growth, and immune responses. Unlike other Gβ subunits, GNB4 exhibits tissue-specific expression, predominantly in the central nervous system, retina, and endocrine tissues, suggesting specialized roles in neural development and sensory functions.
The recombinant protein is typically produced in expression systems like *E. coli* or mammalian cell lines to ensure proper post-translational modifications and functional activity. Purified recombinant GNB4 serves as a vital tool for studying Gβγ-mediated signaling mechanisms, receptor interactions, and downstream effectors such as ion channels and adenylyl cyclases. Dysregulation of GNB4 has been implicated in neurological disorders, retinal degeneration, and cancer, making it a target for therapeutic research. Its WD40 repeat domains, critical for protein-protein interactions, are often studied to uncover structural determinants of signaling specificity. Recombinant GNB4 also aids in high-throughput drug screening and mechanistic studies of diseases linked to GPCR pathway abnormalities.
×
