| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNG3 |
| Uniprot No | P63215 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-75aa |
| 氨基酸序列 | MKGETPVNSTMSIGQARKMVEQLKIEASLCRIKVSKAAADLVTYCDAHACEDPLITPVPTSENPFREKKFFCALL |
| 分子量 | 33.99 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GNG3蛋白的示例参考文献（注：以下内容为模拟示例，实际文献需通过学术数据库检索验证）：
---
1. **文献名称**：**"Cloning, Expression, and Purification of Recombinant Human GNG3 Protein in a Prokaryotic System"**
**作者**：Smith A. et al.
**摘要**：本研究成功克隆了人类GNG3基因，并利用大肠杆菌表达系统实现重组蛋白的高效表达。通过亲和层析纯化获得高纯度蛋白，为后续功能研究奠定基础。
2. **文献名称**：**"Structural Insights into Human GNG3 and Its Role in G Protein Heterotrimer Assembly"**
**作者**：Johnson R.B. et al.
**摘要**：通过X射线晶体学解析重组人GNG3的三维结构，揭示了其与Gβ亚基的相互作用关键位点，阐明了G蛋白异源三聚体组装机制。
3. **文献名称**：**"Functional Analysis of GNG3 in GPCR-mediated Signal Transduction Using Recombinant Protein Knockdown Models"**
**作者**：Lee S. & Park H.
**摘要**：利用重组GNG3蛋白研究其在GPCR信号传导中的功能，发现GNG3缺失会抑制下游效应分子（如cAMP）的活性，表明其在多条信号通路中发挥调控作用。
4. **文献名称**：**"Aberrant Expression of GNG3 in Glioblastoma and Its Impact on Tumor Cell Proliferation"**
**作者**：Chen L. et al.
**摘要**：通过重组GNG3蛋白实验发现，其在胶质母细胞瘤中表达显著上调，且过表达会促进肿瘤细胞增殖，提示其作为潜在治疗靶点的可能性。
---
**提示**：以上为模拟结果，实际文献需通过PubMed、Google Scholar等平台以关键词“recombinant human GNG3”或“GNG3 protein function”检索。
Guanine nucleotide-binding protein gamma-3 (GNG3) is a subunit of heterotrimeric G proteins, which are critical molecular switches in signal transduction pathways mediated by G protein-coupled receptors (GPCRs). As part of the Gβγ complex, GNG3 interacts with Gβ subunits to modulate downstream effectors such as ion channels, enzymes, and other signaling components upon receptor activation. It plays a role in regulating cellular responses to extracellular stimuli, including neurotransmission, hormonal signaling, and sensory perception. Dysregulation of GNG3 has been implicated in various diseases, including cancers, neurological disorders, and metabolic syndromes, underscoring its importance in maintaining cellular homeostasis.
Recombinant human GNG3 protein is engineered using biotechnological platforms (e.g., bacterial, yeast, or mammalian expression systems) to produce highly pure, functional protein for research and therapeutic applications. This engineered protein enables mechanistic studies of Gβγ signaling, GPCR functional assays, and interactions with regulatory proteins or drug candidates. Its use in structural studies (e.g., X-ray crystallography, cryo-EM) has advanced understanding of G protein activation mechanisms. Additionally, recombinant GNG3 serves as a tool for developing targeted therapies aiming to modulate aberrant GPCR signaling in diseases. Tags like His or GST are often fused to facilitate purification and detection. Overall, recombinant GNG3 is pivotal for decoding G protein biology and exploring clinical interventions.
×
