| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GOLGA1 |
| Uniprot No | Q92805 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-767aa |
| 氨基酸序列 | MFAKLKKKIAEETAVAQRPGGATRIPRSVSKESVASMGADSGDDFASDGSSSREDLSSQLLRRNEQIRKLEARLSDYAEQVRNLQKIKEKLEIALEKHQDSSMRKFQEQNETFQANRAKMAEGLALALARKDQEWSEKMDQLEKEKNILTAQLQEMKNQSMNLFQRRDEMDELEGFQQQELSKIKHMLLKKEESLGKMEQELEARTRELSRTQEELMNSNQMSSDLSQKLEELQRHYSTLEEQRDHVIASKTGAESKITALEQKEQELQALIQQLSIDLQKVTAETQEKEDVITHLQEKVASLEKRLEQNLSGEEHVQELLKEKTLAEQNLEDTRQQLLAARSSQAKAINTLETRVRELEQTLQASEEQLQQSKGIVAAQETQIQELAAANQESSHVQQQALALEQQFLERTQALEAQIVALERMRAADQTTAEQGMRQLEQENAALKECRNEYERSLQNHQFELKKLKEEWSQREIVSVAMAQALEEVRKQREEFQQQAANLTAIIDEKEQNLREKTEVLLQKEQEILQLERGHNSALLQIHQLQAELEALRTLKAEEAAVVAEQEDLLRLRGPLQAEALSVNESHVTSRAMQDPVFQLPTAGRTPNGEVGAMDLTQLQKEKQDLEQQLLEKNKTIKQMQQRMLELRKTLQKELKIRPDNELFEVREKPGPEMANMAPSVTNNTDLTDAREINFEYLKHVVLKFMSCRESEAFHLIKAVSVLLNFSQEEENMLKETLEYKMSWFGSKPAPKGSIRPSISNPRIPWS |
| 分子量 | 114.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GOLGA1蛋白的参考文献及相关摘要的整理(注：以下内容基于模拟生成，可能不完全对应真实文献)：
1. **"Structural Insights into Human GOLGA1 and Its Role in Golgi Apparatus Organization"**
- **作者**: Smith J. et al.
- **摘要**: 本研究解析了重组人GOLGA1蛋白的晶体结构，揭示其C端卷曲螺旋结构域介导与高尔基体膜的相互作用，并证明GOLGA1通过调控高尔基体堆叠结构影响细胞内囊泡运输。
2. **"GOLGA1 as a Novel Host Factor for Viral Entry: Interactions with Respiratory Syncytial Virus Glycoprotein"**
- **作者**: Lee S. & Patel R.
- **摘要**: 通过重组人GOLGA1蛋白的体外结合实验，发现其直接结合呼吸道合胞病毒(RSV)的F蛋白，表明GOLGA1在病毒入侵宿主细胞过程中可能作为关键受体或辅助因子。
3. **"Recombinant GOLGA1 Protein as a Biomarker for Primary Ciliary Dyskinesia Screening"**
- **作者**: Gomez F. et al.
- **摘要**: 利用重组GOLGA1蛋白开发了一种酶联免疫吸附检测方法，发现原发性纤毛运动障碍(PCD)患者血清中GOLGA1抗体水平显著升高，提示其作为潜在诊断标志物的价值。
4. **"Functional Characterization of GOLGA1 Knockout Models Using Recombinant Protein Rescue"**
- **作者**: Wang X. et al.
- **摘要**: 在GOLGA1基因敲除细胞系中，通过外源性重组GOLGA1蛋白回补实验，证实其在维持高尔基体形态和细胞分泌功能中的必要性，并揭示与自噬通路的潜在关联。
(注：以上文献信息为示例性质，实际研究中请通过PubMed或学术数据库查询真实文献。)
Recombinant human GOLGA1 protein is a engineered version of the naturally occurring GOLGA1 protein, a member of the golgin family implicated in Golgi apparatus structure and function. GOLGA1. also known as Golgin subfamily A member 1. is characterized by its large coiled-coil domains and localization to the cytoplasmic face of the Golgi. It plays a critical role in maintaining Golgi architecture, facilitating vesicle tethering, and regulating intracellular trafficking by interacting with Rab GTPases and other golgins. Dysregulation of GOLGA1 has been linked to cellular stress responses, cancer progression, and certain genetic disorders affecting organelle organization.
The recombinant form is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells), enabling controlled production of purified, biologically active protein for research. This tool allows scientists to study GOLGA1's molecular interactions, post-translational modifications, and role in diseases like neurodegenerative conditions and carcinomas. Its applications extend to structural studies, antibody development, and screening therapeutic compounds targeting Golgi-related pathways. Recent studies also explore its potential as a biomarker in malignancies where Golgi fragmentation is observed. The availability of recombinant GOLGA1 accelerates mechanistic insights into Golgi dynamics and its broader implications in cellular homeostasis.
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