| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNL3 |
| Uniprot No | Q9BVP2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-549aa |
| 氨基酸序列 | MKRPKLKKASKRMTCHKRYKIQKKVREHHRKLRKEAKKQGHKKPRKDPGVPNSAPFKEALLREAELRKQRLEELKQQQKLDRQKELEKKRKLETNPDIKPSNVEPMEKEFGLCKTENKAKSGKQNSKKLYCQELKKVIEASDVVLEVLDARDPLGCRCPQVEEAIVQSGQKKLVLILNKSDLVPKENLESWLNYLKKELPTVVFRASTKPKDKGKITKRVKAKKNAAPFRSEVCFGKEGLWKLLGGFQETCSKAIRVGVIGFPNVGKSSIINSLKQEQMCNVGVSMGLTRSMQVVPLDKQITIIDSPSFIVSPLNSSSALALRSPASIEVVKPMEAASAILSQADARQVVLKYTVPGYRNSLEFFTMLAQRRGMHQKGGIPNVEGAAKLLWSEWTGASLAYYCHPPTSWTPPPYFNESIVVDMKSGFNLEELEKNNAQSIRAIKGPHLANSILFQSSGLTNGIIEEKDIHEELPKRKERKQEEREDDKDSDQETVDEEVDENSSGMFAAEETGEALSEETTAGEQSTRSFILDKIIEEDDAYDFSTDYV |
| 分子量 | 88.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GNL3蛋白的3篇代表性文献的简要总结（文献标题、作者及摘要内容均基于领域内研究方向综合概括，非真实文献）：
1. **文献名称**：*"Recombinant human GNL3 protein promotes hepatocellular carcinoma cell proliferation by regulating ribosomal RNA synthesis"*
**作者**：Meng, L. et al.
**摘要**：本研究在大肠杆菌系统中成功表达并纯化了重组人GNL3蛋白，证实其可通过结合核仁RNA聚合酶复合体，增强肝癌细胞中rRNA的转录，从而促进肿瘤细胞增殖和化疗耐药性。
2. **文献名称**：*"Structural and functional analysis of the GNL3 nucleolar GTPase domain"*
**作者**：Jia, Y. et al.
**摘要**：利用X射线晶体学解析了重组人GNL3蛋白的GTP酶结构域三维结构，揭示了其核苷酸结合位点的关键氨基酸残基，并通过体外实验证明其GTP水解活性对维持干细胞自我更新能力至关重要。
3. **文献名称**：*"GNL3重组蛋白通过调控p53信号通路抑制结直肠癌细胞凋亡"*
**作者**：Wang, Q. et al.
**摘要**：通过哺乳动物细胞表达系统获得高活性重组GNL3蛋白，实验显示其可结合并抑制p53的核定位，从而阻断细胞凋亡通路，为GNL3在结直肠癌治疗中的靶向干预提供了依据。
*注：以上文献信息为基于研究领域的综合示例，实际文献需通过PubMed或Web of Science等数据库检索，并核实具体作者及发表年份。*
**Background of Recombinant Human GNL3 Protein**
GNL3 (Guanine Nucleotide-Binding Protein-Like 3), also known as Nucleostemin, is a nucleolar protein implicated in cell proliferation, stem cell maintenance, and tumorigenesis. It is highly expressed in embryonic stem cells, cancer cells, and progenitor cells, but downregulated in differentiated tissues. GNL3 plays a critical role in regulating the cell cycle, particularly in G1/S transition, by interacting with p53 and MDM2 to modulate ribosomal RNA synthesis and stress responses. Its dynamic shuttling between the nucleolus and nucleoplasm is essential for maintaining stem cell pluripotency and self-renewal.
Recombinant human GNL3 protein is produced using biotechnological methods, enabling studies on its structure-function relationships and therapeutic potential. Research highlights its involvement in cancer progression, including metastasis and chemoresistance, making it a biomarker and target for oncology. Additionally, GNL3's role in tissue regeneration and aging is under exploration. Structural features like its GTP-binding domain and coiled-coil regions are key to its activity, aiding in drug discovery. Understanding GNL3 offers insights into cellular homeostasis and disease mechanisms.
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