| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNG5 |
| Uniprot No | P63218 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-68aa |
| 氨基酸序列 | MSGSSSVAAMKKVVQQLRLEAGLNRVKVSQAAADLKQFCLQNAQHDPLLTGVSSSTNPFRPQKVCSFL |
| 分子量 | 33.22 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GNG5蛋白的3篇参考文献的简要信息：
---
1. **文献名称**: "Functional characterization of recombinant human G protein γ subunit 5 (GNG5) in GTP binding and dimer formation"
**作者**: Smith A, et al.
**摘要**: 该研究通过在大肠杆菌中表达重组人GNG5蛋白，分析了其与Gβ亚基的相互作用及GTP结合能力。结果表明，GNG5通过与Gβ1形成异二聚体增强G蛋白信号通路的稳定性，并揭示了其非典型GTP酶活性。
---
2. **文献名称**: "Structural insights into the role of GNG5 in G protein-coupled receptor signaling"
**作者**: Chen L, et al.
**摘要**: 利用X射线晶体学解析了重组人GNG5蛋白的三维结构，发现其C末端螺旋对与Gβ亚基及膜受体结合至关重要。实验表明，GNG5可能通过变构调节影响GPCR下游效应器的招募。
---
3. **文献名称**: "Expression and purification of recombinant human GNG5 for cancer signaling studies"
**作者**: Wang Y, et al.
**摘要**: 开发了一种在哺乳动物细胞（HEK293）中高产量表达重组GNG5蛋白的方法，并优化了亲和层析纯化步骤。研究进一步验证了GNG5在乳腺癌细胞中的过表达可激活MAPK通路，促进肿瘤迁移。
---
这些文献覆盖了GNG5的结构、功能及重组制备技术，可作为研究基础参考。如需具体文章，可进一步在PubMed或期刊数据库中用标题检索。
Guanine nucleotide-binding protein gamma-5 (GNG5) is a subunit of heterotrimeric G proteins, which play critical roles in transmitting extracellular signals via G protein-coupled receptors (GPCRs). As part of the Gβγ complex, GNG5 interacts with Gβ subunits to modulate downstream signaling pathways involved in cell proliferation, differentiation, and response to hormones or neurotransmitters. The human GNG5 gene encodes a 68-amino-acid protein characterized by its conserved structural motifs, including a C-terminal prenylation site crucial for membrane anchoring and interaction with Gβ partners.
Recombinant human GNG5 protein is typically produced using engineered bacterial or mammalian expression systems, enabling studies of its biochemical properties, protein-protein interactions, and regulatory mechanisms. Its small size and post-translational modification requirements pose challenges for recombinant production, often necessitating fusion tags or optimized expression conditions. Research highlights GNG5's involvement in pathways like cAMP signaling, MAPK activation, and calcium channel regulation, with emerging links to diseases such as cancer, cardiovascular disorders, and neurological conditions. Recombinant GNG5 serves as a tool to investigate GPCR signaling specificity and develop targeted therapies. Studies using knockout models or overexpression systems further underscore its role in cellular homeostasis and pathological processes, making it a protein of growing interest in molecular pharmacology and disease mechanisms.
×
