| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNG7 |
| Uniprot No | O60262 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-68aa |
| 氨基酸序列 | MSATNNIAQARKLVEQLRIEAGIERIKVSKAASDLMSYCEQHARNDPLLVGVPASENPFKDKKPCIIL |
| 分子量 | 33.22 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的3条关于重组人GNG7蛋白的参考文献示例（实际文献需通过学术数据库查询）：
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1. **文献名称**: *GNG7 inhibits hepatocellular carcinoma progression by blocking mTOR signaling pathway*
**作者**: Liu Y et al.
**摘要**: 研究通过构建重组人GNG7蛋白，发现其过表达可抑制肝癌细胞增殖和侵袭，机制涉及通过mTOR信号通路调节细胞代谢和凋亡。实验验证了GNG7的肿瘤抑制功能。
2. **文献名称**: *Expression and purification of recombinant human GNG7 in E. coli for functional characterization*
**作者**: Zhang R et al.
**摘要**: 报道了一种高效的大肠杆菌表达系统，用于重组人GNG7蛋白的可溶性表达与纯化。通过体外实验证实该蛋白与Gβ亚基结合的能力，并调节MAPK信号通路活性。
3. **文献名称**: *GNG7 suppresses lung adenocarcinoma metastasis via AMPK-mediated cytoskeletal remodeling*
**作者**: Chen H et al.
**摘要**: 研究发现GNG7在肺腺癌中低表达，重组GNG7蛋白通过激活AMPK通路抑制癌细胞迁移和转移，机制涉及对细胞骨架重组的调控，提出其作为潜在治疗靶点。
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**备注**：以上为虚拟参考文献，如需真实数据建议通过 **PubMed（https://pubmed.ncbi.nlm.nih.gov）** 或 **Google Scholar** 搜索关键词如“recombinant GNG7 protein”或“GNG7 cancer signaling”获取。
G protein subunit gamma 7 (GNG7) is a member of the heterotrimeric G protein gamma subunit family, which plays a critical role in intracellular signal transduction. G proteins, composed of α, β, and γ subunits, mediate cellular responses to extracellular signals by coupling transmembrane receptors (e.g., GPCRs) to downstream effectors. GNG7 specifically interacts with Gβ subunits to form the βγ dimer, regulating the activity of various signaling pathways, including ion channels, enzymes, and second messengers.
Human GNG7 is encoded by the GNG7 gene and is expressed in multiple tissues, with notable roles in the brain, cardiovascular system, and endocrine organs. Dysregulation of GNG7 has been linked to pathological conditions such as cancers, hypertension, and metabolic disorders. For instance, reduced GNG7 expression is observed in certain tumors, suggesting its potential as a tumor suppressor by modulating cell proliferation and apoptosis.
Recombinant human GNG7 protein is produced via genetic engineering in systems like *E. coli* or mammalian cells, enabling precise study of its structure-function relationships, interactions with Gβ partners, and regulatory mechanisms in vitro. It serves as a vital tool for elucidating G protein-mediated signaling cascades, screening therapeutic agents targeting G protein-coupled pathways, and exploring disease mechanisms. Researchers also leverage recombinant GNG7 to investigate isoform-specific signaling diversity within the gamma subunit family, advancing our understanding of cellular communication networks.
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