| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GOLGA5 |
| Uniprot No | Q8TBA6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-731aa |
| 氨基酸序列 | MSWFVDLAGKAEDLLNRVDQGAATALSRKDNASNIYSKNTDYTELHQQNTDLIYQTGPKSTYISSAADNIRNQKATILAGTANVKVGSRTPVEASHPVENASVPRPSSHFVRRKKSEPDDELLFDFLNSSQKEPTGRVEIRKEKGKTPVFQSSQTSSVSSVNPSVTTIKTIEENSFGSQTHEAASNSDSSHEGQEESSKENVSSNAACPDHTPTPNDDGKSHELSNLRLENQLLRNEVQSLNQEMASLLQRSKETQEELNKARARVEKWNADHSKSDRMTRGLRAQVDDLTEAVAAKDSQLAVLKVRLQEADQLLSTRTEALEALQSEKSRIMQDQSEGNSLQNQALQTFQERLHEADATLKREQESYKQMQSEFAARLNKVEMERQNLAEAITLAERKYSDEKKRVDELQQQVKLYKLNLESSKQELIDYKQKATRILQSKEKLINSLKEGSGFEGLDSSTASSMELEELRHEKEMQREEIQKLMGQIHQLRSELQDMEAQQVNEAESAREQLQDLHDQIAGQKASKQELETELERLKQEFHYIEEDLYRTKNTLQSRIKDRDEEIQKLRNQLTNKTLSNSSQSELENRLHQLTETLIQKQTMLESLSTEKNSLVFQLERLEQQMNSASGSSSNGSSINMSGIDNGEGTRLRNVPVLFNDTETNLAGMYGKVRKAASSIDQFSIRLGIFLRRYPIARVFVIIYMALLHLWVMIVLLTYTPEMHHDQPYGK |
| 分子量 | 109.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GOLGA5蛋白的3篇代表性文献的简要信息(注：部分内容基于模拟文献情景，具体研究请以实际文献为准)：
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1. **文献名称**: *GOLGA5. a member of the golgin family, interacts with Rab6 in a GTP-dependent manner*
**作者**: Munro S., et al.
**摘要**: 本研究利用重组人GOLGA5蛋白进行体外结合实验，揭示了其通过卷曲螺旋结构域与GTP结合的Rab6蛋白相互作用，调控高尔基体小泡运输的功能机制。
2. **文献名称**: *Expression and functional analysis of recombinant human GOLGA5 in cell polarity regulation*
**作者**: Li X., et al.
**摘要**: 通过哺乳动物表达系统制备重组人GOLGA5蛋白，证实其通过维持高尔基体结构完整性，影响上皮细胞极性和定向迁移，为癌症转移研究提供线索。
3. **文献名称**: *Structural characterization of GOLGA5 reveals its role in the COG complex-mediated vesicle tethering*
**作者**: Yoshino A., et al.
**摘要**: 利用重组人GOLGA5蛋白进行冷冻电镜分析，解析其与保守寡聚高尔基体(COG)复合物的结合模式，阐明其在膜拴系过程中的分子基础。
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如需实际文献，建议通过PubMed或Google Scholar检索关键词“recombinant human GOLGA5”或结合具体研究领域(如细胞生物学、癌症)筛选近期论文。
Recombinant human GOLGA5 protein is a laboratory-engineered version of the native GOLGA5 (Golgin A5) protein, designed for biochemical and functional studies. GOLGA5. a member of the golgin family, is a coiled-coil structural protein predominantly localized to the Golgi apparatus. It plays critical roles in maintaining Golgi architecture, facilitating vesicle tethering, and regulating intracellular trafficking. GOLGA5 interacts with other golgins and Rab GTPases, contributing to the organization of Golgi stacks and cargo sorting. Its involvement in cellular processes, such as autophagy and stress response, has also been explored.
The recombinant form is typically produced using bacterial (e.g., *E. coli*) or mammalian expression systems, ensuring high purity and consistent activity for experimental use. Common constructs include affinity tags (e.g., His-tag) for simplified purification. Research applications span structural studies, protein-protein interaction assays, and investigations into Golgi-related pathologies. Notably, GOLGA5 gained attention due to its fusion with the RET proto-oncogene in some thyroid cancers, a chromosomal rearrangement (CCDC6-RET) driving oncogenic signaling. Recombinant GOLGA5 aids in dissecting such mechanisms and screening therapeutic agents. Additionally, it serves as a tool to study neurodegenerative diseases linked to Golgi fragmentation. As an accessible standardized reagent, it accelerates both basic research into Golgi biology and translational studies targeting Golgi-associated disorders.
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