| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GOLGB1 |
| Uniprot No | Q14789 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 3-91aa |
| 氨基酸序列 | SRLSGLANVVLHELSGDDDTDQNMRAPLDPELHQESDMEFNNTTQEDVQERLAYAEQLVVELKDIIRQKDVQLQQKDEALQEERKAADN |
| 分子量 | 35.53 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假想研究的模拟参考文献示例，实际文献需通过学术数据库查询：
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1. **"Structural insights into the human Golgin GOLGB1 by recombinant protein analysis"**
*Smith, J. et al. (2020). Journal of Molecular Biology*
摘要：通过重组表达人GOLGB1蛋白，解析其N端卷曲螺旋结构域对高尔基体膜锚定的作用，并揭示了与Rab6 GTP酶的相互作用机制。
2. **"GOLGB1 regulates ER-to-Golgi trafficking through interaction with COPI vesicles"**
*Zhang, Y. et al. (2021). Cell Structure and Function*
摘要：研究发现重组GOLGB1蛋白通过结合COPI囊泡组分调控内质网-高尔基体间运输，敲低GOLGB1导致分泌蛋白异常滞留。
3. **"Association of GOLGB1 mutations with skeletal ciliopathies in human"**
*Brown, K. et al. (2019). Human Molecular Genetics*
摘要：利用重组GOLGB1突变体进行功能研究，发现其点突变破坏纤毛发生，导致骨骼发育异常，揭示了GOLGB1在纤毛相关疾病中的潜在作用。
4. **"Proteomic screening identifies GOLGB1 as a novel autophagy regulator"**
*Lee, H. et al. (2022). Autophagy*
摘要：通过重组GOLGB1蛋白的体外互作实验，发现其与自噬相关蛋白LC3的相互作用，调控应激条件下细胞自噬进程。
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**注意**：以上文献为虚构示例，实际研究中GOLGB1相关论文可能较少，建议通过PubMed或Google Scholar以“GOLGB1 recombinant”或“Golgin B1 recombinant human”为关键词检索最新成果。
**Recombinant human GOLGB1 protein** is a engineered form of the Golgin subfamily B member 1 (GOLGB1), a high-molecular-weight peripheral membrane protein predominantly localized to the Golgi apparatus. GOLGB1. also known as golgin-245 or giantin, plays critical roles in maintaining Golgi structure, vesicle trafficking, and glycosylation processes. It acts as a tethering factor for COPI vesicles and participates in Golgi ribbon formation, ensuring proper cargo sorting and organelle integrity. Recombinant GOLGB1 is typically produced in heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies without the complexity of isolating it from native tissues. Researchers use this purified protein to investigate its interactions with Rab GTPases, coiled-coil domains, and other Golgi-associated proteins, as well as to study defects linked to diseases like ciliopathies, cancer, and neurodegenerative disorders. Its recombinant form also aids in antibody production for diagnostic applications. Studies on recombinant GOLGB1 have provided insights into Golgi dynamics during cell division, stress responses, and intracellular signaling. However, challenges remain in expressing full-length protein due to its large size (~3.700 amino acids), often leading to the use of truncated variants for structural and biochemical analyses.
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