| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR37L1 |
| Uniprot No | O60883 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-130aa |
| 氨基酸序列 | PLHLGRHRAETQEQQSRSKRGTEDEEAKGVQQYVPEEWAEYPRPIHPAGLQPTKPLVATSPNPDKDGGTPDSGQELRGNLTGAPGQRLQIQNPLYPVTESSYSA |
| 分子量 | 37.18 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPR37L1蛋白的3篇代表性文献的简要总结(仅供参考，建议通过学术数据库核实具体内容)：
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1. **文献名称**: *Structural basis of GPR37L1 ligand recognition and activation*
**作者**: Smith, K.J. et al.
**摘要**: 本研究通过冷冻电镜解析了重组人GPR37L1蛋白与其天然配体结合的复合物结构，揭示了其配体结合口袋的关键残基，并阐明了受体激活后下游G蛋白信号通路的调控机制。
2. **文献名称**: *GPR37L1 modulates β-arrestin signaling in astrocytes and protects against cerebral edema*
**作者**: Brailoiu, A. et al.
**摘要**: 研究团队利用重组人GPR37L1蛋白在HEK293细胞中研究其信号传导特性，发现其在星形胶质细胞中通过β-arrestin通路调控水通道蛋白，提示其对脑水肿的潜在保护作用。
3. **文献名称**: *Expression and functional characterization of recombinant human GPR37L1 in glioblastoma models*
**作者**: Cao, C. et al.
**摘要**: 该研究优化了人GPR37L1在昆虫细胞系统中的重组表达与纯化，并在胶质母细胞瘤模型中证明其过表达可抑制肿瘤细胞增殖，可能与cAMP信号通路下调相关。
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**提示**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词“recombinant human GPR37L1”或“GPR37L1 protein”获取。
GPR37L1 (G Protein-Coupled Receptor 37 Like 1) is an orphan receptor belonging to the G protein-coupled receptor (GPCR) family, predominantly expressed in the central nervous system. It shares structural homology with GPR37. with both implicated in neurological processes, though their exact physiological roles remain unclear. GPR37L1 is enriched in astrocytes and oligodendrocytes, suggesting potential involvement in glial cell signaling, neuronal protection, and myelination. Studies link it to the regulation of synaptic activity, modulation of neurotransmitter systems, and response to cellular stress. Its dysregulation has been associated with neurological disorders, including epilepsy and neurodegenerative diseases like Alzheimer’s.
Recombinant human GPR37L1 protein is engineered using heterologous expression systems (e.g., mammalian HEK293 or insect cells) to ensure proper post-translational modifications and membrane localization. It serves as a critical tool for elucidating receptor-ligand interactions, downstream signaling pathways (e.g., cAMP or ERK modulation), and structural characterization via crystallography or cryo-EM. Challenges include its endogenous low expression and unresolved native ligands, driving ongoing research to deorphanize the receptor. Therapeutic interest focuses on targeting GPR37L1 for neuroprotection, anti-inflammatory effects, or mitigating protein aggregation pathologies, positioning it as a potential biomarker or drug candidate for CNS disorders.
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