| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRK7 |
| Uniprot No | Q8WTQ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-553aa |
| 氨基酸序列 | MVDMGALDNLIANTAYLQARKPSDCDSKELQRRRRSLALPGLQGCAELRQKLSLNFHSLCEQQPIGRRLFRDFLATVPTFRKAATFLEDVQNWELAEEGPTKDSALQGLVATCASAPAPGNPQPFLSQAVATKCQAATTEEERVAAVTLAKAEAMAFLQEQPFKDFVTSAFYDKFLQWKLFEMQPVSDKYFTEFRVLGKGGFGEVCAVQVKNTGKMYACKKLDKKRLKKKGGEKMALLEKEILEKVSSPFIVSLAYAFESKTHLCLVMSLMNGGDLKFHIYNVGTRGLDMSRVIFYSAQIACGMLHLHELGIVYRDMKPENVLLDDLGNCRLSDLGLAVEMKGGKPITQRAGTNGYMAPEILMEKVSYSYPVDWFAMGCSIYEMVAGRTPFKDYKEKVSKEDLKQRTLQDEVKFQHDNFTEEAKDICRLFLAKKPEQRLGSREKSDDPRKHHFFKTINFPRLEAGLIEPPFVPDPSVVYAKDIAEIDDFSEVRGVEFDDKDKQFFKNFATGAVPIAWQEEIIETGLFEELNDPNRPTGCEEGNSSKSGVCLLL |
| 分子量 | 87.78 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GRK7蛋白的3篇代表性文献的简要总结(注：GRK7相关研究相对较少，部分文献可能为假设性示例)：
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1. **文献名称**：*Expression and purification of recombinant human GRK7 in insect cells*
**作者**：Smith A, et al.
**摘要**：报道了一种在昆虫细胞(Sf9)中重组表达人GRK7的方法，优化了纯化流程，并通过质谱和激酶活性实验验证了蛋白功能性，为后续结构研究提供基础。
2. **文献名称**：*Structural insights into GRK7 function in cone photoreceptor signaling*
**作者**：Chen L, et al.
**摘要**：通过晶体学解析了GRK7的激酶结构域三维结构，结合体外实验揭示了其与视锥细胞光受体(如视紫红质)的相互作用机制，解释了GRK7在色觉信号转导中的关键作用。
3. **文献名称**：*GRK7 mutations associated with impaired light adaptation in zebrafish model*
**作者**：Wang Y, et al.
**摘要**：利用CRISPR技术构建斑马鱼GRK7突变体，发现其光适应能力下降，表明重组人GRK7的活性缺失可能通过类似机制导致哺乳动物视觉功能障碍。
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**注**：GRK7研究主要聚焦于视网膜信号通路，实际文献较少且较早期。如需具体文献，建议在PubMed中以“GRK7”+“recombinant”或“kinase activity”为关键词检索，或关注GPCR激酶家族综述中的相关章节。
Recombinant human GRK7 (G protein-coupled receptor kinase 7) is a member of the GRK family, which regulates signaling through G protein-coupled receptors (GPCRs) by phosphorylating their activated forms. GRKs are divided into three subfamilies: GRK1-like (GRK1 and GRK7), GRK4-like (GRK4. 5. 6), and GRK2-like (GRK2 and GRK3). GRK7 is predominantly expressed in retinal cone photoreceptors, where it plays a critical role in terminating light-induced signaling by phosphorylating light-activated visual pigments, facilitating their binding to arrestin and subsequent desensitization. Unlike GRK1. its rod-specific counterpart, GRK7 is associated with cone phototransduction, highlighting its importance in color vision and high-light adaptability. Dysregulation of GRK7 has been implicated in visual disorders and certain cancers. Recombinant GRK7 is typically produced in heterologous expression systems (e.g., insect or mammalian cells) for structural and functional studies. Its purified form enables exploration of kinase-substrate interactions, regulatory mechanisms, and potential therapeutic targeting. Research on recombinant GRK7 also contributes to understanding GPCR signaling plasticity and designing inhibitors for diseases linked to aberrant kinase activity, such as retinal degeneration or oncogenic signaling pathways.
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