| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HES4 |
| Uniprot No | Q9HCC6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-221aa |
| 氨基酸序列 | MAADTPGKPSASPMAGAPASASRTPDKPRSAAEHRKSSKPVMEKRRRARINESLAQLKTLILDALRKESSRHSKLEKADILEMTVRHLRSLRRVQVTAALSADPAVLGKYRAGFHECLAEVNRFLAGCEGVPADVRSRLLGHLAACLRQLGPSRRPASLSPAAPAEAPAPEVYAGRPLLPSLGGPFPLLAPPLLPGLTRALPAAPRAGPQGPGGPWRPWLR |
| 分子量 | 49.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HES4蛋白的模拟参考文献示例(非真实文献，供格式参考)：
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1. **文献名称**: "Structural and Functional Analysis of Recombinant Human HES4 Protein"
**作者**: Zhang Y, et al.
**摘要**: 本研究通过原核表达系统纯化重组人HES4蛋白，并利用X射线晶体学解析其三维结构，发现其bHLH结构域特异性结合DNA的N-box序列。体外实验证实HES4抑制神经元分化相关基因的转录活性。
2. **文献名称**: "HES4 Regulates Pluripotency in Embryonic Stem Cells via Epigenetic Modifications"
**作者**: Smith J, et al.
**摘要**: 文章通过重组HES4蛋白过表达实验，发现其通过招募组蛋白去乙酰化酶复合物(HDAC)维持胚胎干细胞的未分化状态。ChIP-seq结果显示HES4靶向调控多能性基因OCT4和NANOG的染色质可及性。
3. **文献名称**: "Role of Recombinant HES4 in Hepatocellular Carcinoma Proliferation"
**作者**: Li X, et al.
**摘要**: 研究利用重组HES4蛋白处理肝癌细胞系，发现其通过激活Wnt/β-catenin通路促进肿瘤细胞增殖。临床样本分析显示HES4高表达与患者不良预后显著相关。
4. **文献名称**: "Interaction Between HES4 and Notch Signaling in Neural Development"
**作者**: Tanaka K, et al.
**摘要**: 通过体外神经干细胞模型，探讨重组HES4蛋白对Notch通路的影响。结果表明，HES4与Notch胞内结构域(NICD)形成复合物，增强下游靶基因HES1的表达，调控神经前体细胞分化。
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**注意**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等学术平台检索关键词(如"HES4 recombinant protein"或"hairy and enhancer of split 4")。近年研究可能涉及HES4在癌症、干细胞或神经疾病中的机制。
**Background of Recombinant Human HES4 Protein**
HES4 (Hairy and Enhancer of Split 4) is a member of the HES family of basic helix-loop-helix (bHLH) transcriptional repressors, which are downstream effectors of the Notch signaling pathway. This pathway is evolutionarily conserved and plays a pivotal role in cell fate determination, differentiation, and tissue development. HES4 contains a conserved bHLH domain that facilitates DNA binding to E-box sequences (CANNTG) and interaction with co-repressors like Groucho/TLE proteins, enabling the repression of target genes.
Functionally, HES4 is implicated in maintaining progenitor or stem cell populations by inhibiting premature differentiation. Studies suggest its involvement in neurogenesis, hematopoiesis, and muscle development. Dysregulation of HES4 has been linked to developmental disorders and cancers, where aberrant Notch signaling often drives tumorigenesis. For instance, HES4 overexpression is observed in certain leukemia subtypes and solid tumors, highlighting its potential as a therapeutic target.
Recombinant human HES4 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure proper folding and post-translational modifications. It serves as a critical tool for *in vitro* studies, including binding assays, mechanism exploration, and stem cell differentiation research. By modulating HES4 activity, researchers aim to unravel its role in tissue homeostasis and disease, offering insights into regenerative medicine and anticancer strategies.
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