| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HES3 |
| Uniprot No | Q5TGS1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-186aa |
| 氨基酸序列 | MEKKRRARINVSLEQLKSLLEKHYSHQIRKRKLEKADILELSVKYMRSLQNSLQGLWPVPRGAEQPSGFRSCLPGVSQLLRRGDEVGSGLRCPLVPESAAGSTMDSAGLGQEAPALFRPCTPAVWAPAPAAGGPRSPPPLLLLPESLPGSSASVPPPQPASSRCAESPGLGLRVWRPWGSPGDDLN |
| 分子量 | 20.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几条关于重组人HES3蛋白的模拟参考文献示例(供参考，非真实文献)：
1. **文献名称**：Expression and Functional Characterization of Recombinant Human HES3 Protein in Stem Cell Maintenance
**作者**：Smith A. et al.
**摘要**：该研究报道了通过哺乳动物表达系统成功获得重组人HES3蛋白，并证明其能通过Notch信号通路维持胚胎干细胞的自我更新能力，抑制分化。
2. **文献名称**：Optimization of Recombinant HES3 Production in E. coli and Its Stability Analysis
**作者**：Lee J. et al.
**摘要**：本文优化了重组人HES3蛋白在大肠杆菌中的可溶性表达条件，纯化后检测其二级结构稳定性，为体外功能研究提供高纯度蛋白。
3. **文献名称**：HES3 as a Critical Mediator in Neural Progenitor Cell Fate Determination
**作者**：Gupta R. et al.
**摘要**：利用重组HES3蛋白处理神经前体细胞，发现其通过抑制下游分化基因(如MASH1)促进细胞向胶质细胞谱系分化，而非神经元方向。
4. **文献名称**：Crystal Structure and DNA-binding Mechanism of Recombinant Human HES3 Protein
**作者**：Chen X. et al.
**摘要**：首次解析了重组人HES3蛋白的晶体结构，揭示其碱性螺旋-环-螺旋(bHLH)结构域与靶基因DNA结合的关键位点。
**注意**：以上文献为示例性质，实际研究中建议通过PubMed、Google Scholar等平台检索真实文献(关键词：recombinant HES3、HES3 protein、neural stem cells、Notch signaling)。
Recombinant human HES3 protein is a key regulatory factor involved in stem cell maintenance and differentiation. As a member of the HES (Hairy/Enhancer of Split) family, HES3 functions as a transcriptional repressor that regulates cell fate decisions, particularly in neural progenitor cells. It contains a basic helix-loop-helix (bHLH) domain, enabling DNA binding, and interacts with co-repressors to inhibit the expression of target genes, including proneural genes. HES3 is closely associated with the Notch signaling pathway, a conserved mechanism governing cell proliferation, differentiation, and tissue patterning during development.
The recombinant form of HES3 is produced using expression systems like *E. coli* or mammalian cells, followed by purification to ensure biological activity. This engineered protein retains the functional properties of native HES3. making it valuable for *in vitro* studies on stem cell self-renewal, neural development, and disease modeling. Researchers employ recombinant HES3 to explore its role in maintaining pluripotency, delaying differentiation in culture, or modulating Notch signaling dynamics. Its applications extend to regenerative medicine, drug screening, and understanding neurodevelopmental disorders. However, functional studies require careful optimization due to context-dependent signaling crosstalk and tissue-specific effects.
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