| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H3D |
| Uniprot No | P68431 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-136aa |
| 氨基酸序列 | MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA |
| 分子量 | 42.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人HIST1H3D蛋白研究的代表性文献摘要：
1. **"Expression and purification of recombinant human HIST1H3D protein for epigenetic studies"**
*Author: Lee et al.*
摘要：本研究优化了大肠杆菌中重组HIST1H3D蛋白的表达与纯化工艺，通过His标签亲和层析和凝胶过滤获得高纯度蛋白，并验证其在体外核小体重组实验中的功能，为表观遗传修饰研究提供工具。
2. **"Structural insights into the histone variant HIST1H3D and its interaction with DNA"**
*Author: Zhang et al.*
摘要：利用X射线晶体学解析重组HIST1H3D蛋白与DNA复合物的结构，揭示了该组蛋白与DNA结合的分子机制，发现其C端氨基酸残基在稳定核小体核心中起关键作用。
3. **"HIST1H3D K27M mutation impairs epigenetic regulation in glioblastoma"**
*Author: Smith et al.*
摘要：通过体外重组表达HIST1H3D K27M突变体蛋白，证明该突变显著抑制PRC2复合体的甲基转移酶活性，导致组蛋白H3K27三甲基化水平下降，为胶质母细胞瘤表观遗传失调机制提供实验证据。
注：以上文献为基于领域知识的模拟示例，实际文献检索建议通过PubMed或Web of Science以"HIST1H3D recombinant protein"等关键词进行查询。
The human HIST1H3D gene encodes histone H3.1. a core component of nucleosomes responsible for DNA packaging and epigenetic regulation. As part of the histone H3 family, H3.1 is predominantly expressed during the S phase of the cell cycle and incorporated into chromatin during DNA replication. Unlike variant histones (e.g., H3.3), canonical H3.1 histones like HIST1H3D play critical roles in maintaining chromatin integrity and transmitting epigenetic patterns through cell divisions. Post-translational modifications (e.g., acetylation, methylation) at specific residues (e.g., lysine 4. 9. 27) enable dynamic regulation of gene expression, influencing cellular differentiation and genome stability. Recombinant HIST1H3D protein, produced via bacterial or eukaryotic expression systems, serves as a key tool for studying nucleosome assembly, histone-modifying enzymes, and disease-associated epigenetic dysregulation. It is particularly valuable in cancer research, as mutations in HIST1H3D (notably K27M in diffuse midline gliomas) disrupt chromatin signaling. Additionally, its applications extend to developing inhibitors targeting aberrant histone modifications in neurological disorders and malignancies.
×
