| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST2H2AA |
| Uniprot No | P04908 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-130aa |
| 氨基酸序列 | MSGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYMAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGKVTIAQGGVLPNIQAVLLPKKTESHHKAKGK |
| 分子量 | 40.04 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人HIST2H2AA蛋白的示例参考文献(注：以下为虚构文献示例，建议通过学术数据库查询真实文献)：
1. **文献名称**: "Expression and Purification of Recombinant Human HIST2H2AA in *E. coli* for Chromatin Studies"
**作者**: Szerlong, H.J. et al.
**摘要**: 研究报道了利用大肠杆菌系统高效表达重组人HIST2H2AA蛋白的优化方法，并验证了其与核小体组装相关蛋白的相互作用。
2. **文献名称**: "HIST2H2AA as a Modulator of Epigenetic Regulation in Cancer Cells"
**作者**: Guo, L. et al.
**摘要**: 通过重组HIST2H2AA蛋白的功能实验，揭示了该组蛋白变体在肿瘤细胞染色质重塑和基因沉默中的潜在作用。
3. **文献名称**: "Structural Analysis of Recombinant HIST2H2AA Using X-ray Crystallography"
**作者**: Kato, D. et al.
**摘要**: 解析了重组HIST2H2AA蛋白的三维结构，探讨了其N端尾部的翻译后修饰对DNA结合能力的影响。
4. **文献名称**: "A Novel Role of HIST2H2AA in DNA Damage Response"
**作者**: Mattiroli, F. et al.
**摘要**: 利用重组蛋白体外实验，发现HIST2H2AA通过特定泛素化修饰参与DNA损伤修复信号的传递。
建议通过PubMed、Google Scholar或Web of Science搜索关键词(如“recombinant HIST2H2AA protein”“human histone H2A variant”)获取真实文献。
Recombinant human HIST2H2AA protein is a genetically engineered variant of the HIST2H2AA gene product, a core component of nucleosomes in eukaryotic cells. As a member of the histone H2A family, HIST2H2AA plays a critical role in chromatin structure and epigenetic regulation. It is encoded by the HIST2H2AA gene located on chromosome 1q21.2 within the human histone gene cluster. Unlike replication-dependent histones, HIST2H2AA is classified as a replication-independent variant, suggesting potential specialized functions in transcriptional regulation, DNA repair, or chromatin remodeling during specific cellular processes.
The recombinant protein is typically produced in bacterial (e.g., E. coli) or eukaryotic expression systems to ensure proper folding and post-translational modifications. Its production enables controlled studies on nucleosome assembly, histone-DNA interactions, and epigenetic mechanisms. Researchers employ recombinant HIST2H2AA for investigating histone modification crosstalk (e.g., ubiquitination, phosphorylation), chromatin dynamics in gene expression, and cellular responses to DNA damage. It also serves as an antigen for antibody development and a tool for structural studies using techniques like X-ray crystallography or cryo-EM. Dysregulation of HIST2H2AA has been implicated in cancers and developmental disorders, making this recombinant protein valuable for both basic research and therapeutic target discovery.
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