| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H4G |
| Uniprot No | Q99525 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-98aa |
| 氨基酸序列 | MSVRGKAGKGLGKGGAKCHRKVLSDNIQGITKCTIRRLARHGGVKRILGLIYEETRRVFKVFLENVIWYAVTNTEHAKRKTVTAMAVVYVLKRQGRTL |
| 分子量 | 10.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HIST1H4G蛋白的参考文献(示例为虚构内容，仅供参考格式)：
1. **"Functional characterization of human histone H4 isoforms in chromatin assembly"**
*Smith A, et al.*
摘要：研究比较了H4家族多个亚型(包括HIST1H4G)重组蛋白在体外核小体重组中的作用，发现HIST1H4G在染色质压缩中具有独特效率，可能与异染色质形成相关。
2. **"Recombinant HIST1H4G production and its role in DNA repair"**
*Lee B, Chen X.*
摘要：利用昆虫细胞系统表达重组人HIST1H4G蛋白，并验证其在DNA损伤修复中的功能，揭示其通过与BRCA1复合体相互作用促进修复的分子机制。
3. **"Epigenetic regulation by HIST1H4G acetylation: Insights from recombinant protein assays"**
*Wang Y, et al.*
摘要：通过重组HIST1H4G蛋白研究其乙酰化修饰对组蛋白乙酰转移酶p300的响应，证明特定赖氨酸位点乙酰化调控基因转录活性的差异。
4. **"Structural dynamics of recombinant HIST1H4G in nucleosome assembly"**
*Garcia R, Müller S.*
摘要：利用重组HIST1H4G解析其在核小体中的构象变化，结合分子模拟技术揭示该亚型对染色质动态稳定的贡献。
**注意**：上述文献为示例性质，实际文献需通过PubMed、Google Scholar等平台检索确认。若研究稀缺，建议扩展关键词或关注组蛋白H4家族共性研究。
**Background of Recombinant Human HIST1H4G Protein**
The HIST1H4G gene encodes a member of the histone H4 family, essential components of nucleosomes that organize eukaryotic DNA into chromatin. As a core histone, H4 partners with H3. H2A, and H2B to form an octamer around which DNA wraps, influencing chromatin structure and regulating gene expression. HIST1H4G, located within the HIST1 cluster on chromosome 6. is one of multiple H4 variants, though its specific functional distinctions remain under investigation. Histones undergo post-translational modifications (e.g., acetylation, methylation) that modulate DNA accessibility and cellular processes like transcription, replication, and repair. Recombinant human HIST1H4G protein is produced using biotechnological systems (e.g., *E. coli* or mammalian cells) to enable studies on its structural and functional roles. Its recombinant form allows controlled experimentation, free of cross-reactivity from native cellular modifiers. Research applications include elucidating histone-DNA interactions, epigenetic regulation, and chromatin remodeling mechanisms. Dysregulation of histone H4 variants is linked to cancers and developmental disorders, making HIST1H4G a potential target for therapeutic exploration. The recombinant protein also serves as an antigen for antibody development or a standard in proteomic analyses. Understanding HIST1H4G contributes to broader insights into genome stability and epigenetic diseases.
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